1. Knockdown of NR4A1 induces endoplasmic reticulum stress–mediated apoptosis by increasing ROS production in pancreatic cancer cells.
Knockdown of NR4A1 induces endoplasmic reticulum stress–mediated apoptosis by increasing ROS production in pancreatic cancer cells. A, Panc-1 cells were transfected with each indicated siRNA for 72 hours, and whole cell lysates were analyzed by Western blot analysis. B–D, cells were transfected with siScr or siNR4A1 for 6 hours. At 60 hours after transfection, ROS production was measured by the oxidation of nonfluorescent DCFH-DA to fluorescent DCF using a fluorescence plate reader (B). #P < vs. siScr without reduced GSH. At 24 hours after transfection, the cells were treated with GSH for an additional 48 hours and whole cell lysates were analyzed by Western blot analysis (C and D). β-Actin was used as a loading control. The multiple lanes represent lysates from different experiments.
Syng-Ook Lee et al. Mol Cancer Res 2014;12:
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