1. Ectopic Lin28b functionally replaces CD19 in B cell development and maintenance.
Ectopic Lin28b functionally replaces CD19 in B cell development and maintenance. (A) Representative FACS plots showing frequency of splenic B cell adult mice of the indicated genotypes fed a DOX diet for 3 weeks (n = 5 to 7 from three experiments). (B) Quantification (left) and absolute B cell numbers (right) in spleen of mice from (A). (C) CD5 levels on ABM ImmB cells from (A). (D) Representative plots of κ and λ light chain usage in ABM ImmB cells. (E) Quantification of (D) (n = 4 to 9 from three experiments). (F) Representative Western blot of pPDK1, pGSK3b, c-Myc, human Lin28b (tg-Lin28b), pS6, and b-actin levels in magnetically lineage-depleted (CD3−Gr1−CD11b−Ter119−) splenic B cells (n ≥ 3). (G) Representative histogram of pS6 levels in ABM ImmB cells from WT and tet-Lin28b mice (n ≥ 3). (H) Proposed CD19/c-Myc/Lin28b positive feedback loop in neonatal and adult mice.
Stijn Vanhee et al. Sci. Immunol. 2019;4:eaax4453
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