1. Molecular Spies for Bioimaging—Fluorescent Protein-Based Probes
Atsushi Miyawaki, Yusuke Niino 
Molecular Cell 
Volume 58, Issue 4, Pages (May 2015)
DOI: /j.molcel
Copyright © 2015 Elsevier Inc. Terms and Conditions

2. Figure 1 Schematics of FRET-Based GECIs, YCs
(A) The original YC, including YC3.12.
(B) YC with cp173YFP, including YC3.60.
Molecular Cell  , DOI: ( /j.molcel )
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3. Figure 2 FRET Constructs
(A and B) Bimolecular (A) and unimolecular (B) FRET. D, donor; A, acceptor. Violet, blue, and orange zigzag arrows indicate excitation light for the donor, emission from the donor, and emission from the acceptor, respectively.
Molecular Cell  , DOI: ( /j.molcel )
Copyright © 2015 Elsevier Inc. Terms and Conditions

4. Figure 3 Schematics of Single-FP-Based GECIs
(A) G-CaMP, GCaMP, and pericam.
(B) Camgaroo.
Molecular Cell  , DOI: ( /j.molcel )
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5. Figure 4
Anatomical Distribution of Activity Clusters in One Fish during a Visuomotor Behavior
Top, whole-brain recording of activity using GCaMP5G reveals four temporal clusters.
Bottom, schematic outlining relevant brain regions in the zebrafish larvae in a dorsal view. OT, optic tectum; OT spv, optic tectum stratum periventriculare; IO, inferior olive; Pt, pretectal area; Hab, habenula.
Data adapted from Portugues et al. (2014).
Molecular Cell  , DOI: ( /j.molcel )
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6. Figure 5
Correlation of Cell-Density-Dependent Stochastic ERK Dynamics with Cell Proliferation
(A) Montage FRET images of nuclei in three representative cells expressing nuclear-localized EKAREV with different cell densities.
(B) Frequency of ERK activity pulses as a function of cell density.
(C) Cell-density-dependent proliferation rate in cells analyzed by EdU incorporation.
(D) Percentage of EdU-positive cells as a function of cell density.
Data adapted from Aoki et al. (2013a).
Molecular Cell  , DOI: ( /j.molcel )
Copyright © 2015 Elsevier Inc. Terms and Conditions