1. Evaluation of human sperm function after being cryopreserved within the zona pellucida 
Yinghui Ye, M.D., Ph.D., Chenming Xu, PhD., Yuli Qian, B.Sc., Fan Jin, M.D., Hefeng Huang, M.D. 
Fertility and Sterility 
Volume 92, Issue 3, Pages (September 2009)
DOI: /j.fertnstert
Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Empty zona pellucida without (A) or with (B) injected spermatozoa.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Assessment of sperm chromatin damage using acridine orange (AO) staining. Fluorescence images of spermatozoa from fresh (A) or post-thaw samples cryopreserved with method I (B) or method II (C) stained with AO. Spermatozoa with intact chromatin were stained green and spermatozoa with damaged chromatin were stained orange or red. (D) Effect of cryopreservation on sperm chromatin damage. Values are mean and bars are SEM (n = 15); ∗P<.05 versus control group.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Assessment of sperm DNA fragmentation by terminal deoxynucleotidyl transferase-mediated dUDP nick-end labeling (TUNEL) assay. TUNEL assay applied to spermatozoa from fresh (A) or post-thaw samples cryopreserved with method I (B) or method II (C). Spermatozoa with or without DNA fragmentation showed green and blue fluorescence, respectively. (D) Effect of cryopreservation on sperm DNA fragmentation. Values are mean and bars are SEM (n = 12); ∗P<.05 versus control group.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions