1. Transformation of lsf1 Plants with LSF1 Variants.
Transformation of lsf1 Plants with LSF1 Variants. (A) Schematic diagram of the LSF1 constructs with their domain structures indicated. Point mutations in the DSP and CBM48 domains are marked with a red asterisk, and changes in nucleotide sequences are shown. The constructs were transformed into lsf1 plants, with expression driven by the native LSF1 promoter (PLSF1). (B) Immunoblot analysis of total protein extracts of transformed lsf1 plants. SDS-PAGE gels were loaded on an equal leaf area basis. (C) Representative images of 4-week-old rosettes of the wild type (Col), untransformed lsf1, and complemented lsf1 plants with the wild-type LSF1, LSF1 C390S, and LSF1 W479A W514A expressed under the control of the native promoter. The rosettes were stained with iodine to detect starch. (D) Starch content at the end of day (ED) and end of night (EN) measured in 3-week-old plant rosettes. Values are the mean ± se from six individual plants. Values marked with asterisks (*) are significantly different from the corresponding wild type (Col) using a two-tailed t test at P < The numbers 1 and 2 in (B) and (D) refer to two different homozygous lines for each transgene from independent transformation events.
Tina B. Schreier et al. Plant Cell 2019;31:
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