1. Fetal-derived γδ T cells infiltrate the meninges from birth.
Fetal-derived γδ T cells infiltrate the meninges from birth. Meningeal cell suspensions were prepared from 8- to 20-week-old C57BL/6J WT and Il17aCre R26ReYFP mice (A, B, D, and E), 0- to 52-week-old C57BL/6J WT mice (C), and 20-week-old WT and WT ➔ WT BMC mice (F). Samples were analyzed for the expression of indicated surface (CD45, CD3, TCRδ, CD4, CD8, Vγ1, Vγ4, Vγ5, and Vγ6), markers. Live cells were gated using LiveDead Fixable Viability dye as shown in (A). Dot plots represent cell populations from indicated gates. Histograms depict percentages or absolute numbers from indicated populations. Meningeal spaces were pooled from four mice. Spleens were analyzed from individual mice. Results are representative of four to seven independent experiments. Error bars, mean ± SEM. *P < 0.05, **P < 0.01, and ***P < as calculated by Student’s t test (parametric) or Mann-Whitney U test (nonparametric).
Miguel Ribeiro et al. Sci. Immunol. 2019;4:eaay5199
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