1. Knockdown of ROR1 increases the invasive potential of melanoma cells in vitro and in vivo.
Knockdown of ROR1 increases the invasive potential of melanoma cells in vitro and in vivo. A, ROR1 protein expression examined by Western blot analysis following treatment with CTRL or ROR1 siRNA (using multiple siRNAs) for 72 hours in WM35, WM983B, and WM1799 cells. B, expression of ROR1 following ROR2 knockdown after 48 and 72 hours in cells. C, invasive potential of WM35, WM983B, and WM1799 cells following treatment with ROR1 siRNA assessed by 3D spheroid assays. D, tumor growth assay (in vivo) in WM35 cells following treatment with CTRL or ROR1 siRNA (two different ROR1 siRNAs). E, representative images of lung metastases following treatment with CTRL or ROR1 siRNA. F, graphical representation of counts of metastatic colonies seen in lungs of CTRL, ROR1_5, or ROR1_6 siRNA-treated mice.
Michael P. O'Connell et al. Cancer Discovery 2013;3:
©2013 by American Association for Cancer Research