1. Effect of bile salt on pancreatic lipase activity
Done by :Sahar AlSaubaie

2. Principle:

8. Shake by rotation each of flasks at 15min intervals
Then add 8 drops of phenolphthalein indicator to both flasks and titrate against 0.05 N sodium hydroxide to a distinctly permanent pink (30 sec at least)end point.
Record the titration volumes and calculate the meg. y free fatty acids produced with and without the presence of bile salts.

9. Calculations: C1 X V1 = C2 X V2 Blank B: 0.01 X V1(NaOH) = C2 X 0.5
Sample A:
0.01 X V1 = C2 X 0.5
C2 (blank) – C2 (sample) =Net free fatty acid in
0.5ml of fat