1. SCLC in CMV TKO mice arises from an alternative cell of origin.
SCLC in CMV TKO mice arises from an alternative cell of origin. A, Representative immunofluorescent images of whole-mount staining and confocal imaging of tumors in CMV TKO and CGRP TKO mice. Tumors are circled. Number and percentage of lesions at each location are indicated. T, tumor; B, bronchi; TB, terminal bronchiole; A, alveolar space; SA, streptavidin (which nonspecifically labels lung epithelial cells). Scale bars, 200 μm. B, Representative H&E images of the lungs of mice 3.5 months after transduction by the same titer of adenoviral vectors with distinct promoters driving Cre expression. Scale bars, 1 mm. C, Quantification of SCLC tumor number 3.5 months and 10 months after transduction with the same total titer of different types and combinations of adenoviral vectors with distinct promoters driving Cre expression. Tumor number is quantified from H&E lung sections. The number of mice in each cohort is indicated in parentheses. D, Model of SCLC development and progression from two distinct cell types of origin.
Dian Yang et al. Cancer Discov 2018;8:
©2018 by American Association for Cancer Research