1. Pregnancy Testing-QuickVue One Step
WellOne primary medical and dental care
For Medical Clinical Staff
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2. MA Standing Orders- Pregnancy Testing
Collect and perform urine pregnancy testing on patients who request a pregnancy test, have missed/are late for menses, have irregular vaginal bleeding, pre- IUD or other contraceptive implant insertion, pre-colposcopy, contraception initiation and women of childbearing age with symptoms including but not limited to: pelvic/abdominal pain, nausea, vomiting, etc.
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3. WARNING
Conducting this test exposes the operator to potentially infectious material. Standard precautions, including glove use, are required for this procedure.
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4. QuickVue Pregnancy Testing: Storage and Stability
Store kit at room temperature  F, out of direct sunlight.
Kit contents are stable until the expiration date printed on the outer box carton.
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5. QuickVue Pregnancy Testing
This test detects Human chorionic gonadotropin (hCG) in the urine
hCG is a hormone produced in pregnancy that is made by the embryo soon after conception
This test generally detects levels of hCG as low as 25 mIU/mL
In normal pregnancy, hCG can be detected as early as 6 days following conception with concentrations doubling every 32 to 48 hours. Levels at 25 mIU/mL of hCG (clinically significant pregnancy level) are reportedly present in serum and urine as early as 2-3 days before expected menses.
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6. QuickVue Pregnancy Testing
First morning urine specimens generally contain the highest levels of hCG and may yield a more accurate test.
If the urine is very dilute and has a low specific gravity (a specific gravity of ) , the sample may not contain representative urinary hCG concentrations and may fall below the detection level of our test. If this is suspected, it is suggested that another sample be used, ideally first morning void.
Refrigerate specimen if unable to test within eight (8) hours. Let specimen and test return to room temperature before testing.
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7. QuickVue Pregnancy Testing:
The contents of this kit are of use in the qualitative detection of HCG in urine only.
This test shall be used as a definitive test. However, when pregnancy is strongly suggested on clinical grounds and the urine test is negative, venous laboratory testing shall be ordered for confirmation.
While pregnancy is the most likely reason for the presence of hCG in serum and urine, elevated hCG concentrations unrelated to pregnancy have been reported in some patients. Conditions other than normal pregnancy may be associated with detectable hCG, including, for example, ectopic pregnancy or molar pregnancy.
hCG may remain detectable for a few days to several weeks after delivery, abortion, or hCG injections. Abnormal pregnancies cannot be diagnosed by qualitative HCG results. The above conditions should be ruled out when diagnosing pregnancy. Test results must always be evaluated with other data available to the clinician. As with all laboratory tests, definitive diagnostic or therapeutic decisions should not be based on any single result or method but should be based on a correlation of test results with typical clinical signs and symptoms.
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8. Testing should not be performed on urine more than 8 hours old unless:
the urine has had aspirin added to it to
preserve it.
the urine has been refrigerated and
allowed to return to room temperature
prior to testing
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9. The correct answer is:
the urine has been refrigerated and allowed to return to room temperature
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10. QuickVue Pregnancy Testing: General Steps
Identify patient using two patient identifiers (name and DOB)
Explain procedure to patient in culturally appropriate, age appropriate terms.
Gather all necessary equipment and supplies prior to start of the procedure.
Inspect all equipment and supplies for damage, general integrity and expiration date- do not use any damaged or expired materials
Perform hand hygiene before and after patient contact; before and after donning gloves.
Don all appropriate personal protective equipment prior to conducting procedure.
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11. expiration date and general integrity
The pregnancy test device should be inspected for __________ prior to use:
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expiration date and general integrity
lot number
control line
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12. ……….expiration date and general integrity
The pregnancy test device should be inspected for __________ prior to use:
……….expiration date and general integrity
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13. QuickVue Pregnancy Testing: Procedural Steps
Remove test device from the protective pouch and place it on a clean, dry, flat surface.
Label the device with patient initials and DOB.
Using one of the disposable pipettes supplied with the kit, add 3 drops of urine into the round sample well on the Test Cassette. The Test Cassette should not be manipulated or jarred again until the assay is complete and ready for interpretation. Wait for colored lines to appear.
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14. The QuickVue urine pregnancy test used at WellOne requires:
6 drops of urine 2 drops of urine 3 drops of urine
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15. The correct answer is…………………..
3 drops of urine
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16. QuickVue Pregnancy Testing: Procedural Steps
Read results after 3 minutes and no later than 30 minutes.
A control zone © is built-in to the Quick Vue. The development of the blue procedural Control Line next to the letter “C” is a positive procedural control. If this line does not develop, the test result is invalid. The absence of interfering background is a negative procedural control. If background color appears in the Result Window which interferes with your ability to read the test result, your result may be invalid. In this case, contact COO or designee.
The test is NEGATIVE if the blue procedural Control Line next to the letter “C” appears only and no pink-to-purple Test Line next to the letter “T” appears at three minutes.
The test is POSITIVE if any pink-to-purple line next to the letter “T” in the Result Window, along with a blue procedural Control Line next to the letter “C” appears.
Dispose of test in regular trash.
Remove gloves and place in regular trash.
Perform hand hygiene.
Document results in patient's record. Document results in EMR.
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17. 3 minutes, 30 minutes 3 minutes, 40 minutes 3 minutes, 60 minutes
Test results should be read at ______ minutes and no later than _________ minutes
3 minutes, 30 minutes
3 minutes, 40 minutes
3 minutes, 60 minutes
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18. The correct answer is……
Test results should be read at ________ minutes …………………….. and no later than _________ minutes
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19. A pink to purple line next to the letter “T” means that the test:
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Is not valid
Is negative
Is positive
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20. The correct answer is: The test is positive
A pink to purple line at the “T” (test) indicates a positive test whereas the lack of a line indicates a negative test
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21. If a blue line does not appear next to the “C” on the test:
the test is positive
the test is negative
the test is not valid and another test
must be run
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22. The correct answer is: the test is not valid and another test must be run
A vivid blue line at the “C” (control) means that the test is acceptable. A lack of a vivid blue line means there is something wrong with the test cassette and the test must be repeated.
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23. You have reached the end of this program…..
Once you are confident that you are comfortable with all the materials presented, proceed to the post-test at \
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