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Early pediatric atopic dermatitis shows only a cutaneous lymphocyte antigen (CLA)+ TH2/TH1 cell imbalance, whereas adults acquire CLA+ TH22/TC22 cell.

Published byJonathan Madsen Modified over 5 years ago

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Presentation on theme: "Early pediatric atopic dermatitis shows only a cutaneous lymphocyte antigen (CLA)+ TH2/TH1 cell imbalance, whereas adults acquire CLA+ TH22/TC22 cell."— Presentation transcript:

1 Early pediatric atopic dermatitis shows only a cutaneous lymphocyte antigen (CLA)+ TH2/TH1 cell imbalance, whereas adults acquire CLA+ TH22/TC22 cell subsets  Tali Czarnowicki, MD, MSc, Hitokazu Esaki, MD, Juana Gonzalez, PhD, Dana Malajian, BA, Avner Shemer, MD, Shinji Noda, MD, Sreya Talasila, MD, Adam Berry, MS, Jayla Gray, BS, Lauren Becker, MD, Yeriel Estrada, BS, Hui Xu, MSc, Xiuzhong Zheng, MSc, Mayte Suárez-Fariñas, PhD, James G. Krueger, MD, PhD, Amy S. Paller, MD, Emma Guttman-Yassky, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 136, Issue 4, Pages e3 (October 2015) DOI: /j.jaci Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 CD4 (A) and CD8 (B) T-cell subsets in children and adults with AD versus control subjects. Naive T-cell frequencies were higher in both control subjects and children with AD compared with those in adults, whereas TCM/TEM cell subsets were lower in control children and children with AD than adults. Adults with AD had significantly higher numbers of effector cells compared with those in children with AD. *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 ICOS expression in CLA+ and CLA− memory T-cell subsets in adults and children with AD. A, Patients with AD had increased ICOS expression in both CLA+ and CLA− memory cell subsets compared with their relative control subjects. No significant CD4 ICOS expression differences were observed between the AD groups in both CLA+/CLA− TEM/TCM cell subsets. B, Contrary to adults, in which higher CD8 ICOS expression was measured in CLA+ TCM/TEM cells compared with their relative control subjects, no differences were observed between children with AD and control subjects. *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 HLA-DR and CD69 expression in CLA+ and CLA− memory T-cell subsets in adults and children with AD. A, Comparable levels of HLA-DR expression were seen on TCM/TEM cells between children with and without AD. B, Adults with AD had higher HLA-DR expression compared with that of control subjects, particularly among the CLA+ and CD8 cell populations. C, Lower CD69 cells in adults than in children with AD in both CD4 and CD8 subsets. *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 IFN-γ and IL-13 polarization in CLA+ and CLA− CD4 and CD8 T cells. A, Compared with children, adults with AD had significantly higher IFN-γ levels, particularly in CD4 T cells. IFN-γ+CLA+ cell numbers in children with AD were significantly lower than in control children. B, In adults IL-13 levels were higher in the AD group compared with control values in both CLA+/CLA− CD4/CD8 cell subsets. In children this difference was most prominent among CD4 CLA+ T cells. C, Children with AD had markedly lower TH1/TH2 ratios compared with control subjects, particularly in CD4 CLA+ cells. *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 IL-22, IL-17, and IL-9 frequencies in CLA+ and CLA− CD4 and CD8 T cells. A, No significant IL-22 differences were measured between children with and without AD. B, TH17/TC17 levels were higher in adults versus children with AD, specifically in the CLA− CD4/CD8 cell subsets. C, Adults with AD had higher TH9 levels in CLA+ cells than children with AD. *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E1 Gating strategy and frequency of IFN-γ, IL-13, and IL-22 versus CLA positivity in CD4+ T cells in pediatric and adult healthy control subjects and representative patients with AD. A, Gating strategy. FSC, Forward scatter; SSC, side scatter. B, Representative plots of IFN-γ, IL-13, and IL-22 and CLA expression, showing reduced IFN-γ+CLA+ cells in patients with AD accompanied by increased IL-13+ cell percentages in both children and adults and enhanced IL-22+ cell percentages only in adults with AD. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E2 CD4 and CD8 TCM and TEM cell subset correlations with IgE levels and disease severity/SCORAD scores. A, No correlations were found between TCM/TEM memory cell subsets and IgE levels among control children. B, Positive correlations were observed in children with AD. C, TCM/TEM cell subsets were not correlated with IgE levels in adults with AD. D and E, SCORAD scores were not correlated with TCM/TEM cell frequencies in either children or adults with AD. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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