1. Fig. 2 Dcr-2 is required for Toll activation in adult flies.
Dcr-2 is required for Toll activation in adult flies. (A and B) Six hours after the M. luteus challenge, total RNA extracts were prepared from adult flies with the indicated genotypes and treatment and subjected to Northern blots using the indicated probes (A) or to qRT-PCR of Dros mRNA (normalized to Rp49; n = 3; *P < 0.05, two-tailed Student’s t test; error bars, SD; each group contains 10 female flies and 10 male flies) (B). CyO, second chromosome balancer with curly wings marker. (C) Survival of adult flies with the indicated genotypes after A. fumigatus infection (n = 3; each group contains 10 female flies and 10 male flies; error bars, SD). Flies that died within 3 hours of infection were not considered in the analysis. (D and E) Total RNA extracts were prepared from adult flies with the indicated genotypes and subjected to Northern blots using the indicated probes (D) or to qRT-PCR of Dros mRNA (normalized to Rp49; n = 3; *P < 0.05, two-tailed Student’s t test; error bars, SD) (E).
Zhaowei Wang et al. Sci Adv 2015;1:e
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