1. Proteus mirabilis can utilize d-serine as a sole source of carbon and nitrogen.
Proteus mirabilis can utilize d-serine as a sole source of carbon and nitrogen. Proteus mirabilis strain HI4320 was cultured in LB broth overnight and diluted 1:100 into Proteus minimal salts medium (PMSM) containing increasing concentrations of d-serine as the sole source of carbon (A) or nitrogen (B). Growth was assessed at 37°C with aeration in a 96-well plate reader by measurement of OD600 at hourly intervals for 18 h. Error bars represent means ± standard deviations (SDs) from at least 6 replicates, and graphs are representative of 4 independent experiments. The addition of ≥0.5 mM d-serine as a carbon source or ≥0.05 mM d-serine as a nitrogen source promoted significant growth as determined by paired two-way ANOVA with Dunnett’s test for multiple comparisons (P < 0.001).
Aimee L. Brauer et al. mSphere 2019; doi: /mSphere