1. Optimizing human semen cryopreservation by reducing test vial volume and repetitive test vial sampling 
Christian F.S. Jensen, B.Sc., Dana A. Ohl, M.D., Walter R. Parker, M.D., Andre M. da Rocha, Ph.D., Laura M. Keller, A.A.S., Timothy G. Schuster, M.D., Jens Sonksen, M.D., Ph.D., Gary D. Smith, Ph.D. 
Fertility and Sterility 
Volume 103, Issue 3, Pages e1 (March 2015)
DOI: /j.fertnstert
Copyright © Terms and Conditions

2. Figure 1
Test vial mean ± SEM cryosurvival at multiple volumes. a,b,cDifferent letters are significantly different (P<.05).
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © Terms and Conditions

3. Figure 2
Long-term evaluation (mean ± SEM). (A) Post-thaw motility, (B) progressive motility, and (C) cryosurvival of samples stored in VN2 for donor 1 (2004–2008) and donor 2 (2009–2011).
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © Terms and Conditions

4. Supplemental Figure 1
Long-term evaluation. (A) Recording of the temperature at the top and middle of the VN2 tank. (B) Monthly recording of liquid nitrogen height and usage at the bottom of the VN2 tank.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © Terms and Conditions