1. Maturation of human ovarian follicles is accompanied by a decrease in the CD56+CD16+ natural killer cell population 
Ofer Fainaru, M.D., Ph.D., Shay Hantisteanu, M.Sc., Nechami Rotfarb, B.Sc., Medeia Michaeli, Ph.D., Mordechai Hallak, M.D., Adrian Ellenbogen, M.D. 
Fertility and Sterility 
Volume 95, Issue 7, Pages (June 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Follicular fluid natural killer (NK) cell populations as gated by flow cytometry. (A) Dot plot showing the CD45-high leukocyte population present in follicular fluid derived from a patient undergoing IVF (R1). The CD45− non-gated cells reflect the abundant granulosa cell population. (B) CD3+ T cells were gated out (R2). (C) In the CD45+CD3− gate (R1 and R2), two distinct NK cell populations are shown: CD45+CD3−CD56+CD16− (“angiogenic”) and CD45+CD3−CD56+CD16+ (“cytotoxic”). Compared with peripheral blood, in which there was a predominance of the “cytotoxic” subset of CD56+CD16+ NK cells, follicular fluid was highly enriched for the “angiogenic” CD56+CD16− NK cells.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Natural killer (NK) cell subpopulation analysis in IVF versus IVM patients. The distribution of NK cell subpopulations in follicular fluid derived from a mature follicle of a good-responding IVF patient and that of an antral follicle derived from an IVM patient. Note the predominance of “cytotoxic” CD56+CD16+ NK cells in the antral follicle.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Follicular fluid natural killer (NK) subpopulations in antral versus mature ovarian follicles. The comparison between NK subpopulations in antral (n = 10) versus mature ovarian follicles (n = 22) reflects a ∼3-fold decrease in “cytotoxic” CD56+CD16+ (P=0.001) NK cells and a slight decrease in “proangiogenic” CD56+CD16− (P=0.003) NK cells in the mature follicles.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions