1. The Peroxisomal Transporter PXA1 Is Involved in Ubiquinone Biosynthesis from Phenylalanine.(A) Total ubiquinone (reduced and oxidized) levels in the leaves of wild-type and pxa1 knockout Arabidopsis plants.
The Peroxisomal Transporter PXA1 Is Involved in Ubiquinone Biosynthesis from Phenylalanine.(A) Total ubiquinone (reduced and oxidized) levels in the leaves of wild-type and pxa1 knockout Arabidopsis plants. Data are means of four biological replicates ± se. Asterisk annotations indicate significant difference from the wild type as determined by Fisher’s test (P < α = 0.05) from ANOVA. FW, fresh weight.(B) Total ubiquinone-[Ring-13C6] levels in axenically grown wild-type and pxa1 knockout Arabidopsis plants fed with 100 μM doses of Phe-[13C9] or Tyr-[13C9;15N] for 3 h. Data are means of four biological replicates ± se. Asterisk annotations indicate significant difference from the wild type as determined by Fisher’s test (P < α = 0.05) from ANOVA.(C) Total ubiquinone levels in axenically grown wild-type and pxa1 knockout Arabidopsis plants fed for 24 h with 200 μM t-cinnamate, p-coumarate, or 4-hydroxybenzoate (4-HB). Data are means of three to five biological replicates ± se. Asterisk annotations indicate significant difference from the corresponding DMSO control as determined by Fisher’s test (P < α = 0.05) from ANOVA.(D) Phylloquinone levels in the leaves of wild-type pxa1 Arabidopsis plants. Data are means of three biological replicates ± se. Wild-type and pxa1 values are not significantly different as determined by Fisher's test from ANOVA (P > 0.4).
Anna Block et al. Plant Cell 2014;26:
©2014 by American Society of Plant Biologists