1. Bb monocolonization enhances Treg population in the cLP.
Bb monocolonization enhances Treg population in the cLP. (A) Localization analysis of Bb in the intestinal niches of GF mice by HISTO-FISH staining with DNA-Cy5 probes (EUB338, red) 3 weeks after colonization. (B) Representative flow cytometry plots and percentage analyses of CD4+Foxp3+ cells in cLP of GF mice colonized with indicated bacterial strains. (C to F) Representative flow cytometry plots and frequencies of CD103+ and CD62LloCD44hi, CTLA4+, and IL10+ in Treg cells in GF mice or those monocolonized with Lpa or Bb. Numbers in the quadrants represent cell percentage, and circles in the graph plots represent individual mouse corresponding to each parameter. Data are representative of three to five independent experiments with similar results (n ≥ 3 mice). All graph plots show means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < (Student’s t test). MFI, mean fluorescence intensity; ns, not significant.
Ravi Verma et al. Sci. Immunol. 2018;3:eaat6975
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