1. Intratumoral spatial distribution of granulocytes and T cells.
Intratumoral spatial distribution of granulocytes and T cells. Tissue studio composer technology and image-based machine learning (A), pan-cytokeratin staining (B), and the manual tracing wizard of tissue studio were used to define tumor and stroma regions. Multiparameter immunofluorescence was used to determine the density of CD3 and CD66b cells in 2D tissue sections (n = 137 patients) (C) or whole-mount stained 3D material (n = 11) (F) obtained from patients with HNC. (D) and (E) represent still images of videos and depict CD3 cells (blue), CD66b cells (green), and tumor surface area. Movie S1 in (E) is additionally provided as supplementary material. Using the split into surface Spots tool of Imaris, the density of CD3+ and CD66b+ cells within defined distances to the tumor margin was determined (G to J). Paired t test was used to determine statistical significance.
Yu Si et al. Sci. Immunol. 2019;4:eaaw9159
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