Presentation is loading. Please wait.

Presentation is loading. Please wait.

Confirmation of spliced RNA in cells transfected with a wild-type or Pol(−) MR766 ZIKV plasmid. Confirmation of spliced RNA in cells transfected with a.

Published bySara Irene Hubbard Modified over 5 years ago

Similar presentations

Presentation on theme: "Confirmation of spliced RNA in cells transfected with a wild-type or Pol(−) MR766 ZIKV plasmid. Confirmation of spliced RNA in cells transfected with a."— Presentation transcript:

1 Confirmation of spliced RNA in cells transfected with a wild-type or Pol(−) MR766 ZIKV plasmid.
Confirmation of spliced RNA in cells transfected with a wild-type or Pol(−) MR766 ZIKV plasmid. (A) RT-PCR products from RNA from cells transfected with the wild-type or Pol(−) MR766 ZIKV plasmid or infected with the parental MR766 virus. Band sizes (kilobases) are indicated to the left of the gel. Two product sizes were observed, representing the unspliced (1,083-bp) and spliced (1,216-bp) template. (B) Sequence results of RT-PCR products from transfected or infected cells aligned to three reference sequences: parental MR766 virus, unspliced plasmid, and spliced plasmid. Megan C. Schwarz et al. mSphere 2016; doi: /mSphere

Download ppt "Confirmation of spliced RNA in cells transfected with a wild-type or Pol(−) MR766 ZIKV plasmid. Confirmation of spliced RNA in cells transfected with a."

Similar presentations

Supplemental Figure 2. (A) AtplaIVA-1 and AtplaIVA-2 null transcription lines for AtPLAIVA mRNA. RNAs from the relevant wild type Col were isolated.

Supplemental Figure 2. (A) AtplaIVA-1 and AtplaIVA-2 null transcription lines for AtPLAIVA mRNA. RNAs from the relevant wild type Col were isolated.
Htr-9 stimulates ICAM-1 gene expression in human ASM cells.

Htr-9 stimulates ICAM-1 gene expression in human ASM cells.
A Common Human β Globin Splicing Mutation Modeled in Mice

A Common Human β Globin Splicing Mutation Modeled in Mice
PCR genotype analysis to determine RNP-mediated knockout efficiency in C. lusitaniae. PCR genotype analysis to determine RNP-mediated knockout efficiency.

PCR genotype analysis to determine RNP-mediated knockout efficiency in C. lusitaniae. PCR genotype analysis to determine RNP-mediated knockout efficiency.
In Vivo Tropism of Hepatitis C Virus Genomic Sequences in Hematopoietic Cells: Influence of Viral Load, Viral Genotype, and Cell Phenotype by Hervé Lerat,

In Vivo Tropism of Hepatitis C Virus Genomic Sequences in Hematopoietic Cells: Influence of Viral Load, Viral Genotype, and Cell Phenotype by Hervé Lerat,
Activation of multiple cryptic donor splice sites by the common congenital afibrinogenemia mutation, FGA IVS4 + 1 G→T by Catia Attanasio, Philippe de Moerloose,

Activation of multiple cryptic donor splice sites by the common congenital afibrinogenemia mutation, FGA IVS4 + 1 G→T by Catia Attanasio, Philippe de Moerloose,
Wolbachia bacteria suppress A

Wolbachia bacteria suppress A
Expression and regulation of MD-2s.

Expression and regulation of MD-2s.
Figure 3 Transcripts of the splicing mutation (c

Figure 3 Transcripts of the splicing mutation (c
Supplementary Figure 1 RT-PCR BclxL mismatch sc35 Dox BclxS

Supplementary Figure 1 RT-PCR BclxL mismatch sc35 Dox BclxS
NGS on SOP-generated HRV-16-specific sequence from pure and mixed samples is slightly less sensitive than quantitative real-time RT-PCR (qrRT-PCR). NGS.

NGS on SOP-generated HRV-16-specific sequence from pure and mixed samples is slightly less sensitive than quantitative real-time RT-PCR (qrRT-PCR). NGS.
De novo generation of infectious ZIKVNatal by CPER

De novo generation of infectious ZIKVNatal by CPER
Identification, description, and expression of E7-less STING isoforms.

Identification, description, and expression of E7-less STING isoforms.
ZIKV infection induces DNA methylation changes in human cerebral organoid-derived astrocytes, neurons, and neural progenitor cells. ZIKV infection induces.

ZIKV infection induces DNA methylation changes in human cerebral organoid-derived astrocytes, neurons, and neural progenitor cells. ZIKV infection induces.
Girish C Shukla, Richard A Padgett  Molecular Cell 

Girish C Shukla, Richard A Padgett  Molecular Cell 
Size profile of viral small RNAs obtained from control cells and RIG-I- and MDA5-deficient (KO) cells after infection for 24 h with SINV at an MOI of 0.1,

Size profile of viral small RNAs obtained from control cells and RIG-I- and MDA5-deficient (KO) cells after infection for 24 h with SINV at an MOI of 0.1,
Marc Spingola, Manuel Ares  Molecular Cell 

Marc Spingola, Manuel Ares  Molecular Cell 
RAD51 is essential for L. donovani.

RAD51 is essential for L. donovani.
Adapted viruses replicate in mismatched miR-122-expressing cells.

Adapted viruses replicate in mismatched miR-122-expressing cells.
Functional validation of RIG-I- and MDA5-deficient cell line generated by CRISPR-Cas9 technology. Functional validation of RIG-I- and MDA5-deficient cell.

Functional validation of RIG-I- and MDA5-deficient cell line generated by CRISPR-Cas9 technology. Functional validation of RIG-I- and MDA5-deficient cell.

Similar presentations

Ads by Google