1. Figure 1 A B C Cell media alone Growth factors- Resting Mast cells
FcR1-activated Mast cells
TGF
FGF2 B
Activated MC CM
Anti-TGFb1
Albuterol (10 µM)
min
Albuterol (10 µM) C
GM-CSF
HGF
IGFII ** *

2. Figure 2 A A B C D FGF2 TGFβ1 - - Albuterol +FGF2 +TGFβ1 (10 μM)
Isotype
Basal A A
FGF2
Isotype
Basal B
FGF2
TGFβ1 -
% of positive p-β2-AR (Tyr141) over basal
FGF2
TGFβ1 -
% of positive p-β2-AR (Tyr350) over basal
TGFβ1
Isotype
Basal
TGFβ1
Isotype
Basal C D
+FGF TGFβ1
10 ng/ml ng/ml
Albuterol
(10 μM)

3. Figure 3 B A C D Healthy Mild asthma Severe asthma P-Tyr141 b2-AR
(GMFI)
P-Tyr350 b2-AR
(GMFI)
Healthy
Mild asthma
Severe asthma C D
Healthy controls
Non-severe asthma
Severe Asthma
P value
Number (n) 14 7 -
Age (y)
38.14±4.71
47.85±4.01
55.44±3.54
0.0263
Sex (F/M)
8/6
4/2
5/2
ICS (µg)
225±91.02
±173.7
OCS (no.) 1 3
FEV1 (% predicted)
85.73±4.9
104.75±6.27
74±8.18
0.3189
FEV1/FVC ratio (%)
78.74±1.45
75.82±4.66
62.28±4.27
0.0046
PC20 (mg/ml)
>16
4.82±2.04
0.54±0.464