1. Flow cytometry (fluorescence-activated cell sorting) analyses determined the ploidy of the various somatic hybrid strains.
Flow cytometry (fluorescence-activated cell sorting) analyses determined the ploidy of the various somatic hybrid strains. (A) Schematic representing intralineage spheroplast fusion between J118 and RM1000 generating homotetraploid fusion products CAT1 and CAT2. (B) Schematic representing interlineage spheroplast fusion between UBWP17 and WUM5A generating homotetraploid fusion product CAT3. (C) Schematic representing interspecies spheroplast fusion between J118 and CdUM4b generating heterotetraploid fusion products HBT1 and HBT2. The ploidy of all the parents and their respective hybrid strains was determined by fluorescence-activated cell sorter analyses (right). For ploidy analysis, cells were stained with PI to examine the cellular DNA content. The x axis of each graph (PI) represents a linear scale of fluorescence, and the y axis (counts) represents a linear scale of cell number.
Uttara Chakraborty et al. Eukaryotic Cell 2013; doi: /EC