Presentation is loading. Please wait.

Presentation is loading. Please wait.

Fig. 4 Rotavirus induces the type I IFN pathway and release of ATP by tumor cells. Rotavirus induces the type I IFN pathway and release of ATP by tumor.

Published byJocelyn Atkinson Modified over 5 years ago

Similar presentations

Presentation on theme: "Fig. 4 Rotavirus induces the type I IFN pathway and release of ATP by tumor cells. Rotavirus induces the type I IFN pathway and release of ATP by tumor."— Presentation transcript:

1 Fig. 4 Rotavirus induces the type I IFN pathway and release of ATP by tumor cells.
Rotavirus induces the type I IFN pathway and release of ATP by tumor cells. (A) List of the 23 shared genes commonly up-regulated by A20 and EMT6 cancer cell lines upon in vitro rotavirus exposure with at least 1.5 log2 fold change in expression and a P < (mean values of triplicates). For the two log2 fold change columns, a gradient from white to red allows identification of the genes with the weakest (white) to the strongest (red) up-regulations. Genes highlighted in gray are in common with the list of genes up-regulated in A20 upon exposure with inactivated rotavirus (see Fig. 7D). (B) Pictures of immunofluorescence staining (magnification, ×20) with anti-MxA–specific monoclonal antibodies and secondary detection on A20 tumors harvested from Balb/c mice after either IT rotavirus or IT PBS treatment. Staining was performed on frozen tumor sections. An isotype control was used as a negative control for MxA detection (in green) after DAPI (4′,6-diamidino-2-phenylindole) counterstaining (in blue). The means (±SEM) of immunofluorescence intensity as measured by the ImageJ software are presented in the right panel. Data from n = 2 mice in each group with three tumor sections. Unpaired one-tailed t test, **P = (C) ATP release in the cell culture supernatant upon several dilutions of rotavirus exposure on human (SH-SY5Y neuroblastoma) or murine (Neuro2a neuroblastoma and A20 B-cell lymphoma) tumor cell lines. Measurement performed by luminescence assay using CellTiter-Glo Luminescent Cell Viability Assay kit 48 hours after incubation with different dilutions of rotavirus. Results are reported as RLU intensity corresponding to relative ATP release intensity. Data as means ± SEM, n = 3 wells per dilution; one-way ANOVA test, ****P < , ***P < , and **P < Tala Shekarian et al., Sci Transl Med 2019;11:eaat5025 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works

Download ppt "Fig. 4 Rotavirus induces the type I IFN pathway and release of ATP by tumor cells. Rotavirus induces the type I IFN pathway and release of ATP by tumor."

Similar presentations

Identification of combination treatment–responsive effector/memory tumor-infiltrating CD8+ T cell population. Identification of combination treatment–responsive.

Identification of combination treatment–responsive effector/memory tumor-infiltrating CD8+ T cell population. Identification of combination treatment–responsive.
Identification of combination treatment–responsive dysfunctional tumor-infiltrating CD8+ T cell population. Identification of combination treatment–responsive.

Identification of combination treatment–responsive dysfunctional tumor-infiltrating CD8+ T cell population. Identification of combination treatment–responsive.
Three different types of transfer functions with a codomain of [0,1].

Three different types of transfer functions with a codomain of [0,1].
Resistance of CD141+ DCs to influenza virus in vivo in humanized mice.

Resistance of CD141+ DCs to influenza virus in vivo in humanized mice.
Fig. 3 CSF1 is expressed in human melanoma.

Fig. 3 CSF1 is expressed in human melanoma.
Protection from autoimmunity is not due to the expansion of Treg subsets. Protection from autoimmunity is not due to the expansion of Treg subsets. (A.

Protection from autoimmunity is not due to the expansion of Treg subsets. Protection from autoimmunity is not due to the expansion of Treg subsets. (A.
Platelets are required for hFcγRIIA-induced anaphylaxis.

Platelets are required for hFcγRIIA-induced anaphylaxis.
Human cells produce type I and III IFNs upon Af stimulation.

Human cells produce type I and III IFNs upon Af stimulation.
β-Glucans do not modulate epithelial IL-33 or AHR.

β-Glucans do not modulate epithelial IL-33 or AHR.
Direct visualization by RNA FISH of TLR7 escape from X inactivation in B cells from women. Direct visualization by RNA FISH of TLR7 escape from X inactivation.

Direct visualization by RNA FISH of TLR7 escape from X inactivation in B cells from women. Direct visualization by RNA FISH of TLR7 escape from X inactivation.
Fig. 7 Correlation of NHP and human ISGs.

Fig. 7 Correlation of NHP and human ISGs.
Fig. 5 Putative latency-reversing agents exert differential effects on the different blocks to HIV transcription. Putative latency-reversing agents exert.

Fig. 5 Putative latency-reversing agents exert differential effects on the different blocks to HIV transcription. Putative latency-reversing agents exert.
Differentiation of AZD4785 from MAPK pathway inhibitors in vitro

Differentiation of AZD4785 from MAPK pathway inhibitors in vitro
Fig. 6 In utero injection of inflammatory cytokines or adoptive transfer of activated T cells leads to pregnancy loss. In utero injection of inflammatory.

Fig. 6 In utero injection of inflammatory cytokines or adoptive transfer of activated T cells leads to pregnancy loss. In utero injection of inflammatory.
IL-10R-deficient macrophages secrete IL-23, inducing IL-22 secretion by ILC3 and TH17 cells. IL-10R-deficient macrophages secrete IL-23, inducing IL-22.

IL-10R-deficient macrophages secrete IL-23, inducing IL-22 secretion by ILC3 and TH17 cells. IL-10R-deficient macrophages secrete IL-23, inducing IL-22.
Mammary cell proliferation is inhibited when LAD1 is depleted.

Mammary cell proliferation is inhibited when LAD1 is depleted.
Fig. 4 Labeling of Msmeg with DMN-Tre is fast and specific and depends on Ag85A function. Labeling of Msmeg with DMN-Tre is fast and specific and depends.

Fig. 4 Labeling of Msmeg with DMN-Tre is fast and specific and depends on Ag85A function. Labeling of Msmeg with DMN-Tre is fast and specific and depends.
Fig. 2 AcPGP induces IL-8 and G-CSF release from human bronchial epithelial cells. AcPGP induces IL-8 and G-CSF release from human bronchial epithelial.

Fig. 2 AcPGP induces IL-8 and G-CSF release from human bronchial epithelial cells. AcPGP induces IL-8 and G-CSF release from human bronchial epithelial.
Cell viability tests. Cell viability tests. SEM images of (A) MC3T3-E1 cells and (B) MSCs on days 1, 3, and 5 of culture. (C) Survival rates of MC3T3-E1.

Cell viability tests. Cell viability tests. SEM images of (A) MC3T3-E1 cells and (B) MSCs on days 1, 3, and 5 of culture. (C) Survival rates of MC3T3-E1.
Fig. 4. PVSRIPO infection of DCs is sublethal, is marginally productive, and induces sustained proinflammatory cytokine production. PVSRIPO infection of.

Fig. 4. PVSRIPO infection of DCs is sublethal, is marginally productive, and induces sustained proinflammatory cytokine production. PVSRIPO infection of.

Similar presentations

Ads by Google