1. Fig. 3 4n hybrids undergo bipolar and tripolar mitosis.
4n hybrids undergo bipolar and tripolar mitosis. (A) Immunofluorescence images of control ESCs and 4n hybrids in metaphase (DNA, white; β-tubulin, red). Scale bars, 5 μm. (B) Quantification of bipolar and tripolar metaphase spindles in control ESCs, stable 4n, and mixed clones. (ESC: N = 121 cells, two replicates; stable 4n clones: N = 358 cells, two different clones; mixed clones: N = 377 cells, four different clones; mean ± SD). Exact P value is also indicated. (C) Representative time-lapse images of tetraploid cells from mixed clones undergoing bipolar (top) and tripolar (bottom) mitosis (time progression in minutes is shown on each frame). Scale bars, 10 μm. See also movies S12 and S13 (including bright-field images). Schematic representations of the mitotic outcomes are shown on the right side of each panel. (D to F) Quantification of the centrosome number in bipolar metaphases of stable (D) and mixed clones (E) and of tripolar metaphases in mixed clones (F) (mean ± SD). The number of quantified metaphases (n) is indicated in each graph.
João Frade et al. Sci Adv 2019;5:eaax4199
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