1. Fig. 3 Genome editing of the MSTN gene.
Genome editing of the MSTN gene. (A) Genomic structure of the MSTN locus and sgRNA sequences targeting the first MSTN exon. (B) Representative genomic sequences of blastocysts formed after zygote electroporation with Cas9 protein and MSTN sgRNAs (sgRNA1, sgRNA6, and sgRNA7; see also fig. S2). Arrowheads indicate the Cas9 cleavage sites. (C) Frequency of mutations in the PCR amplicons of each target region. (D) Alignment of sequences from each blastocyst after electroporation. Nucleotides in blue indicate target sequences, and nucleotides in red indicate PAM sequences. Nucleotides in green indicate inserted sequences. WT, wild type.
Fuminori Tanihara et al. Sci Adv 2016;2:e
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