1. Surface expression of chemokine receptors on M
Surface expression of chemokine receptors on M. bovis–specific peripheral γδ and CD4 T cells.
Surface expression of chemokine receptors on M. bovis–specific peripheral γδ and CD4 T cells. PBMCs were isolated from calves ∼8 wk after challenge with virulent M. bovis. Cells were stained with CellTrace dye and incubated with PPD-b for 6 d. Flow cytometry was used to study CXCR3 and CCR5 expression on M. bovis–specific CD4 and γδ T cells. Cells were surface stained and then analyzed by flow cytometry for CXCR3 and CCR5 expression. (A) Representative histograms of CXCR3 and CCR5 expression on Ag-responsive (blue solid line) and nonresponsive CD4 and γδ T cells (red solid line). Cumulative results from live γδ (B) and CD4 T cells (C), gated based on response to M. bovis (i.e., proliferating cells) and analyzed for CXCR3 or CRR5 expression. The fluorescence minus one (FMO) control is shown in gray shaded histograms. Data are presented as mean ± SEM mean fluorescence intensity (MFI) expression of cell surface markers. Results are representative of one independent experiment with n = 10. *p < 0.05, ***p < indicate a significant difference (p < 0.05) from Ag-responsive cells compared with nonresponsive cells as determined by Student t test.
Mariana Guerra-Maupome et al. ImmunoHorizons 2019;3:
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