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Making sense of a gene fusion event in Proteobacteria

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Presentation on theme: "Making sense of a gene fusion event in Proteobacteria"— Presentation transcript:

1 Making sense of a gene fusion event in Proteobacteria
Dawn Anderson (Berea College) Rob Barber (U of Wisconsin Parkside) Teresa Johnson (College of Wooster)

2 A promiscuous protein domain (and coding region)
The GAT-1 family is comprised of proteins containing a Type-1 glutamine amidotransferase-like domain. Proteins in this family display an alpha/beta hydrolase fold with a nucleophilic elbow. This transferase-like domain can be present alone or as a fusion with several other protein domains. A fusion between this domain and an AraC DNA binding domain has been found in prokaryotes.

3 Domain fusion to ArgF DNA binding domain restricted to Proteobacteria
Mesorhizobium sp. Alpha proteobacteria Sinorhizobium meliloti Agrobacterium tumefaciens Silicibacter sp. Magnetospirillum magnetotacticum Paracoccus denitrificans Rhodobacter sphaeroides Polaromonas sp. Beta proteobacteria Chromobacterium violaceum Ralstonia solanacearum Ralstonia metallidurans Ralstonia eutropha Burkholderia vietnamiensis Burkholderia pseudomallei Azotobacter vinelandii Gamma proteobacteria Photobacterium profundum Chromohalobacter salexigens Pseudomonas putida Pseudomonas aeruginosa Vibrio

4 Phylogeny suggests divergence prior to speciation

5 N-terminal domain phylogeny

6 Gene associations/neighborhoods offer little functional inference

7 Approaches to test gene product function
Clone gene, express and purify predicted gene product (trying heparin chromatography) Structure determination and model building exercises Gene expression experiments possible (microarrays, proteomics) but likely offer little insight due to prediction of regulatory role

8 FusionDB offered little insight regarding this protein family

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