1. Gene regulation in biological responses:
Torunn Bruland; post doc NTNU, DMF
Gene regulation in biological responses:
Focus on gastrin responses in neuroendocrine cells in the gastro-intestinal tract

2. Studies of early retrovirus-host interactions
Viral determinants for pathogenesis
and the influence of sex on the susceptibility to
Friend murine leukaemia virus infection
Dr. philos thesis 2003; NTNU, Faculty of Medicine
ANIMAL MODEL (mice)
virology
immunology
cell biology
signal transduction
molecular biology
statistic

3. Gene regulation in biological responses

4. Molecular mechanisms in the normal and
diseased gastrointestinal system
Normal Early gastric cancer Adenocarsinoma

5. Gene regulation in biological responses:
Focus on gastrin responses in neuroendocrine cells in the gastro-intestinal tract
Enterochromaffin-like (ECL) cells are a
distinctive type of neuroendocrine cells in the
gastric mucosa underlying the epithelium
Introduction
Longer term stimulation of ECL cells
by gastrin stimulates significant
proliferation of ECL cells

6. Several gastrin responsive genes contain cAMP responsive
promotor element (CRE)

7. Modulation of gastrin-mediated gene expression
by transcriptional regulators of the CREB/CREM/ATF family

8. Work plan: cDNA/oligo/splice variant MIKROARRAY
gastric carsinoma
gastric carcinoids
hypergastrinemia
gastrin-mediated gene expression
CREB/CREM/ATF-modulated
gene expression
Genome-wide gene expression
gastric mucosa custom microarrays
system-wide screening of CREB/CREM/ATF splice variants

9. Generation of models

10. Reverse Transfection RNAi + Microarray
Hypothesis testing: high throughput/single gene
Reverse Transfection RNAi + Microarray

11. Summary gastrin-mediated gene expression CREB/CREM/ATF-modulated
High throughput
96-well
array based
Single gene
knock-up
knock-down
(RNAi)
animal models
Genome-wide gene expression
gastric mucosa custom microarrays
system-wide screening of CREB/CREM/ATF
splice variants
statistical methods for:
preprocessing and discrimination and classification
rule-based methods for classification
optimal use of biological background knowledge

12. Gene regulation in biological responses:
Focus on gastrin responses in neuroendocrine cells in the gastro-intestinal tract
Prosjektansvarlig
Astrid Lægreid, professor, dr.scient.,
Prosjektmedarbeidere
Liv Thommesen, førsteam.. dr.philos., HiST
Torunn Bruland, post.doc. dr.philos., IKM, NTNU
Ola Ween, post.doc. dr.scient., IKM, NFR
Kristine Misund, cand.scient. NTNU
Tonje Strømmen, siv.ing. NTNU