1. Figure 1 Renilla Luc Tk pRL-Tk Firefly Luc LexA pL6NLuc NRE pLexA-p65 p65LexA MPSV pLexA-p65 pL6NLuc NRF expression vectors - - NRF(1-380) NRF(1-690) - + + + + +

2. Figure 2 p65 RHD p65 endogenous p65 B NRF(1-380)TAP + p65 NRF(1-380)TAP + p65RHD NRF(1-380)TAP NRF(1-380)TAP + p65 NRF(1-380)TAP + p65RHD total bound p65 bound p50 bound A

3. Control NRF-Strep 1 26 51 76 1 26 51 76 Spots B spot peptide sequence 1 1 MDELFPLIFPAEPAQ 15 2 4 LFPLIFPAEPAQASG 18 3 7 LIFPAEPAQASGPYV 21 4 10 PAEPAQASGPYVEII 24. 1 26 51 76 RRL A Figure 3 85 114 199 246 274 309

4. A spot peptide sequence 1 88 HELVGKDC 95 2 89 ELVGKDCR 96 3 90 LVGKDCRD 97 21 87 PHELVGKDC 95 22 88 HELVGKDCR 96 23 89 ELVGKDCRD 97 42 86 HPHELVGKDC 95 43 87 PHELVGKDCR 96 44 88 HELVGKDCRD 97. peptide length 8 aa 9 aa 10 aa.. Figure 4 NRF-Strep 26 51 76 1 101 126 151 176 spots peptide length 8-9 aa 8 9 10 11 12 13 14 15 9-10 aa 10-11 aa 11-12 aa 12-13 aa 13-14 aa 14-15 aa 15 aa B 81 PPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQ 114

5. Figure 5 1 26 Spots NRF-Strep B spot peptide sequence 1 199 VNRNSGSCLGGDEIF 213 2 200 NRNSGSCLGGDEIFL 214 3 201 RNSGSCLGGDEIFLL 215. 199 VNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHR 246 A

6. Figure 6 A 274 RPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTY 306 spot peptide sequence 1 274 RPSDRELSEPMEFQY 288 2 274 APSDRELSEPMEFQY 288 3 274 RASDRELSEPMEFQY 288 4 274 RPADRELSEPMEFQY 288 18 277 DRELSEPMEFQYLPD 291 19 277 ARELSEPMEFQYLPD 291 20 277 DAELSEPMEFQYLPD 291 21 277 DRALSEPMEFQYLPD 291... B NRF-Strep spots 26 51 76 1 101