1. A effervescent tablet disinfectant
SUNGIL PHARMA
A concern of
A effervescent tablet disinfectant
AQUAGARD-T
Good Effervescent !
Long Lasting !
High Effectiveness !
Very Convenient !
Varied Application !

2. Features of Aquagard-T
Good Effervescent !
Long Lasting !
High Effectiveness !
Very Convenient !
Varied Application !

3. Aquagard-T… Aquagard-T is a effervescent tablet disinfectant.
Aquagard-T is a very powerful disinfectant containing sodium dichloroisocyanurate (NaDCC) widely applied for the sterilization of drinking water, or fighting against infectious diseases, or act as disinfectant in raising livestock, poultry and fish.

4. Aquagard-T…
Aquagard-T is an oxidising compound which releases chlorine to produce hypochlorous acid and hypochlorite ion in the presence of water.
It is generally considered that the lethal action on organism is due to chlorination of cell protein or enzyme systems by nonionised hypochlorous acid, causing hydrolysis of peptidic chains of cellular membranes of pathogenic germs. Chlorine-releasing substances are bactericidal to most gram-positive and gram-negative bacteria, bacterial spores and viruses.

5. NaDCC (Sodium Dichloroisocyanurate)
(Structural formula of NaDCC)

6. NaDCC (Sodium Dichloroisocyanurate)
+ 2HOCl
Hypochlorous acid
+ 2H2O
germicidal activity
H+ + OCl-
Hypochlorite ion
(Hydrolysis of NaDCC)

7. Aquagard-T : NaDCC (Sodium Dichloroisocyanurate)
The germicidal activity of hypochlorous acid is 80 times higher than hypochlorite ion.
The presence of HOCl in solution of chlorine containing compound
pH of NaOCl solutions
pH of Aquagard-T solutions

8. Chlorine containing compound
Chlorine used widely as a disinfectant and bleaching agent
Inorganic compound Sodium hypochlorite (NaOCl)
Calcium hypochlorite (CaOCl)
Chloline dioxide (ClO2)
unstable, less germicidal activity
Organic compound Sodium dichloroisocyanurate (NaDCC)
very stable, predominantly germicidal activity

9. Features of Aquagard-T
Good effervescent high technique for making effervescent tablets
Long lasting free available chlorine is present for days in Aquagard-T solutions
High effectiveness broad spectrum disinfectant (active to spore forming bacteria, naked virus)
Very convenient easy to handle and safe
Varied application used widely for disinfection of poultry, pig, cattle and sheep, livestock houses and facilities, drinking water

10. Microbiocidal activity of various disinfectants
Very Sensitive
Sensitive
Resistant
Very Resistant
Septic bacteria
(Pasteurella sp)
Enveloped virus
(HC, TGE,
ILT, IB, ND)
Non-enveloped virus
(Enterovirus,
Adenovirus, FMD,
IBD, Reo)
Spore forming bacteria
(B. anthracis,
Clostridium)
Weak Disinfectants
surfactant,
quaternary ammonium chloride
Intermediate Disinfectants
cresol, phenol,
ortho, alcohol
Strong Disinfectants
chlorine, iodine,
Aldehydes,
ethylene oxide

11. Microbiocidal activity of Aquagard-T
Microbials
NaDCC* (ppm)
Staphylococcus aureus
1.12
Escherichia coli
0.83
Salmonella typhi
Klebsiella pneumoniae
2.36
Serratia marcescens
1.14
Yersinia enterocolitica
0.72
Pseudomonas aeruginosa
2.00
Saccharomyces cerevisiae
72.0
Candida albicans
45.9
* Total available chlorine

12. Bactericidal activity of Aquagard-T
Phenol coefficiency of Aquagard-T against bacteria
Test strains
Phenol coefficiency
Staphylococcus aureus 209P
28.5
Salmonella pullorum 9-25
25.5
Erysipelothrix rhusiopathiae
16.3
Erysipelothrix rhusiopathiae fujisawa

13. Bactericidal activity of Aquagard-T
Effectiveness of Aquagard-T against spore forming bacteria
Disinfectants
Spore
Bacillus subtilis ATCC9372
Bacillus (natto) BN strain
B. Cereus RIMD
Cl. Perfringens Type B NCTC3182
Aspergillus oryzae
Aquagard-T
1:152(5)
1:57(15)
1:380(5)
1:950(15)
1:3,800(15)
1:3,420(15)
Iodine
1:10(30)
<1:10(30)
1:10(15)
<1:10
1:1,000(15)
Benzalkonium chloride
1:150(5)
1:100(5)
1:50(5)
1:1,500(15)
Amphoteric
surfactant
1:50(15)
1:20(5)
1:25(15)
1:10(5)
<1:50(15)
Chlorhexidine
1:200(5)
1:300(15)
1:100(15)
1:1,200(15)
Effective dilution rate, ( ) : Exposure time

14. Bactericidal activity of Aquagard-T
The sporicidal activity of disinfectants
Test strains
NaDCC
NaOCl
Remarks
Bacillus cereus
17.2
1,060
>62 times
Bacillus subtilis
30.9
1,510
>49 times

15. Virucidal activity of Aquagard-T
Virucidal activity against poultry viruses
Adenovirus
IBD virus
ILT
virus ND
NaDCC ○
Iodine ×
Benzalkonium chloride
○ : inactivated, × : not inactivated

16. Virucidal activity of Aquagard-T
Virucidal activity against pig viruses
FMD
virus
Adeno
JE virus
TGE
Aujeszky’s disease virus
Rota virus
HC virus
NaDCC ○
Iodine ×
○ : inactivated, × : not inactivated

17. Fungicidal activity of Aquagard-T
Test fungi
MIC (mcg/ml) of Aquagard-T
Candida albicans
2.6
Candida krusei
26.3
Aspergillus niger
263.2
Aspergillus fumigatus
Penicillium citrinum
Trichophyton verrucosum
Microsporum canis
Trichophyton mentagrophytes

18. The efficacy test scope
Total bacterial colony counts on foot bath in entrance of farm
Disinfectants
Chlorine contents or dilution rate
Elapsed days (day) 1 3 5
Aquagard-T
418ppm -
2.0×103
NaOCl
2.4×105
1.7×105
Quaternary ammonium chloride
1:200
7.0×102
8.3×105
Iodine
1:250
4.0×101
7.0×103
Orthodichlorobenzene
8.7×103
Control
5.0×104
2.0×108
3.2×107
Unit : CFU/ml, - : <1×101CFU/ml

19. Bactericidal activity
Method : According to regulation of NVRQS
Experiment : Research Institute for Veterinary Science in Konkuk Univ.
Results :
Disease
Condition
Light water
Organic substance
Salmonella spp.
1:7,200
1:540
Brucella spp.
1:3,500
1:400
Streptococcus spp.
1:8,000
1:720
It was tested by konkuk University according to the government regulation.
As the result, lifeline is very strong bactericidal activity.
Even in the(디) organic substance,
The dilution rate is two hundred for salmonella.

20. Inclusion Hepatitis virus
Virucidal activity
Method : According to regulation of NVRQS
Experiment : Research Institute for Veterinary Science in Gyeongsang National Univ. and Cheonbuk National Univ.
Results :
Disease
Condition
Light water
Organic substance
Hog cholera virus
1:12,000
1:1,000
PRRS virus
1:12,500
1:750
New castle virus
1:9,000
Avian Influenza Virus
1:15,000
1:500
Avian Pneumovirus
1:700
1:50
Inclusion Hepatitis virus
1:100
It was tested by Seoul National University
according to the government regulation.
As the result, lifeline is very strong virucidal activity.
Even in the organic substance,
The dilution rate is two hundred for ND virus.
And five hundred for avian influenza virus.

21. Composition of Aquagard-T
Each tablet (13g) contains
5g of Sodium dichloroisocyanurate
(= 3g of available chlorine)
and
Component A
(effervescent agent)
Component B
(pH controlling agent)

22. Application of Aquagard-T
Disinfection of virus and bacteria in livestock housing, farms (floor, wall), facilities and equipment, the animal body
Drinking water treatment
Teat dip or spray (mastitis prevention)
Disinfection of pathogen
General bacteria (Salmonella spp.), Brucella spp., Streptococcus spp. and so on.
Foot and Mouth Disease virus, Hog Cholera virus, PRRS virus, Newcastle Disease virus, Avian Influenza Virus, Avian Pneumovirus, Inclusion Body Hepatitis virus and so on

23. Use of Aquagard-T (Bacteria)
Recommended Dilution Rate
Disinfection Target
Low
Contamination Group
High
Livestock building, the surface of the animal's body, equipment, General automobiles
The Floor of the Livestock building, Manure,
the body of the animal
(dead or alive), Automobiles/Vehicles
General Bacteria
(Salmonella spp.)
1:7,200
(1tablet per 93L of water)
1:540
(1tablet per 7L of water)
Brucella spp.
1:3,500
(1tablet per 45L of water)
1:400
(1tablet per 5L of water)
Streptococcus
spp.
1:8,000
(1tablet per 104L of water)
1:720
(1tablet per 9.3L of water)
* Dilution rate is the most effective dilution rate of results of determination of the efficacy of disinfectant against general bacteria and specific pathogen.

24. Use of Aquagard-T (Virus)
Recommended Dilution Rate
Disinfection Target
Low Contamination Group
High Contamination Group
Livestock building, the surface of the animal's body, equipment, General automobiles
The Floor of the Livestock building, Manure, the body of the animal (dead or alive), Automobiles/Vehicles
Hog Cholera virus
1:12,000
(1 tablet per 156L of water)
1:1,000
(1 tablet per 13L of water)
PRRS virus
1:12,500
(1 tablet per 163L of water)
1:750
(1 tablet per 9.7L of water)
ND virus
1:9,000
(1 tablet per 117L of water)
Avian Influenza Virus
1:15,000
(1 tablet per 195L of water)
1:500
(1 tablet per 6.5L of water)
Avian Pneumovirus
1:700
(1 tablet per 9L of water)
1:50
(1 tablet per 650ml of water)
Inclusion body hepatitis virus
1:100
(1 tablet per 1.3L of water)
* Dilution rate is the most effective dilution rate of results of determination of the efficacy of disinfectant against general bacteria and specific pathogen.

25. Use of Aquagard-T (Water Treatment)
Dissolve Aquagard-T in water tank every other day or every day if necessary.
Dilute 1 tablet per 500 ~ 750 L of drinking water according to water quality and farm condition.

26. Use program for poultry
Poultry houses and facilities
- Thorough cleaning with water
- Spray Aquagard-T solution not less than 2 times
before placement of chicks.
- Spray Aquagard-T solution during growing
period if necessary.

27. Use program for poultry
Foot bath
- For disinfect boots by dipping before entering each house, replace the Aquagard-T solutions every 5 days or sooner when soiled by heavy use.
Transport
- For prevention of disease transmission through trucks and crates by disinfecting them with Aquagard-T.
- Use in wheel baths and renew every 5 days or according to volume of traffic.

28. Use program for dairy Dairy houses and facilities
- Thorough cleaning with water
- Spray Aquagard-T solution, eliminate all bacteria,
viruses and fungi.

29. Use program for dairy to prevent Mastitis
Preparation of disinfection
Dissolve 1 Aquagard-T tablet per 1L of water and move to dipping cup or sprayer for disinfection.
Prior to milking
- Clean milk starts with clean teats.
- Teat and udder spraying will remove all bacteria, viruses and fungi.
After milking
- Teat dip or spraying will
decontaminate the teats and prevent
mastitis

30. Use program for Milking
Dipping method
Spray method
1) Disinfection prior to milking
- Removal of contaminant on teat
2) Removal of disinfectant
- With clean and disinfected towel
3) Prior to milking
- More than 5 times
4) Massage and final cleaning
- With clean and disinfected towel
5) Attaching the milking mashing
- Vacuum-blocking
6) Disinfection after milking
- Prevention of teat canal

31. Package of Aquagard-T 10 tablets / plastic container
100 tablets (1.3kg) / plastic container
2,000 tablets / carton

32. Storage and shelf-life of Aquagard-T
Keep under cool and dry conditions and
protect from light
Shelf-life
24 months after manufacturing

33. Efficacy Test Report

34. Effect of Aquagard-T on E. Coli in Water

35. Precautions of Aquagard-T
Keep out of reach of children.
Do not dissolve in strong alkali (over pH 10) or acidic (below pH 3) solution.
Do not exceed the recommended dosage.
For 5 days, from before 2 days to after 2 days of vaccination, do not disinfect drinking water.
Do not place near fire or strong inflammables.
Do not place in metallic vessel more than 2 hours.
In case of drink diluted solution, immediately seek for medical advice.

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