1. The proline accumulation in salt tolerant plants Elferra Swart, Maarten Weerenbeck en David van Diepen

2. Proline Amino acid + compatible osmolyte –Remain unchanged, H 2 O-soluble, no effect on other molecules First plants response water stress: –decrease growth rate and turgor + prevention of feedback inhibition proline synthesis. (Crawford, 1989) protects membranes and proteins against dangers of dehydration Accumulation in cytosol leaves in salt tollerant plants, but also other plants during salt stress..

3. Hypothesis Proline accumulates when salt concentration becomes higher

4. Method Proline can be made visible by reaction with ninhydrine (  purple complex) Extinction muesurement, ED 520 nm Making standard line with known concentration Calculation proline amount

5. Results

9. Discussion No standard measures, but clear accumulation In every plant, proline accumulates, what’s the difference? Tollerant plants don’t begin at 0! Excellent results! –Hypothese correct without doubt Further questions: –Does proline keeps rising? –What kind of trendline between salt and proline? –What is the correlation with other data?

10. Conclusion This was a very interesting talk You can all applaud for me If you really want, I can do the talk again Thank you!

11. Credits: Deze powerpoint is gemaakt door: –David van Diepen Kijk voor meer powerpoints op: –www.davidvandiepen.nlwww.davidvandiepen.nl Ik vind het leuk als u een berichtje achterlaat op mijn gastenboek!