1. Rinse & Chill Technology Novel Intervention for reduction of undesirable bacteria on beef carcasses and On-going Protection from coliforms and E. coli in vacuum packaged ground beef

2. Rinse & Chill Technology
Developed by MPSC, Inc. – St. Paul, MN
Post bleed vascular technology
Involves vascular rinse and chill of carcasses with a solution of saccharides, sodium chloride and phosphates

3. Rinse & Chill Technology
Provides for statistically “cleaner” carcass samples with respect to aerobic microorganisms when compared with controls
2 h cooler sponge samples/3 sampling dates
APC N
Ave.
CFU/cm2
% Reduction
P value
(95% - .05)
Control 90
1224
R&C
731
40.3%
.039*

4. Rinse & Chill Technology
Provides for statistically “cleaner” carcass samples with respect to coliforms microorganisms when compared with controls
2 h cooler sponge samples/3 sampling dates
Coliforms N
Ave.
CFU/cm2
% Reduction
Frequency
P value
(95% - .05)
Control 90 97
22/90
R&C
0.7
99.3%
11/90
0.125

5. Control vs. Rinse & Chill
CFU/cm2
C = Control
T = R&C

6. Rinse & Chill Technology
Provides for statistically “cleaner” carcass samples with respect to aerobics microorganisms when compared with controls
24 h cooler sponge samples/3 sampling dates
APC N
Ave.
CFU/cm2
% Reduction
P value
(95% - .05)
Control 50
580
R&C
341
41.2%
.009*

7. Rinse & Chill Technology
Provides for statistically “cleaner” carcass samples with respect to coliforms microorganisms when compared with controls
24 h cooler sponge samples/3 sampling dates
Coliforms N
Ave.
CFU/cm2
% Reduction
Frequency
P value
(95% - .05)
Control 50
345
41/50
R&C
111
67.8%
31/50
0.002*

8. Rinse & Chill Technology
Provides for statistically “cleaner” carcass samples with respect to generic E. coli microorganisms when compared with controls
24 h cooler sponge samples/3 sampling dates
Generic
E. coli N
Ave.
CFU/cm2
% Reduction
Frequency
P value
(95% - .05)
Control 50
294
31/50
R&C 48
83.7%
16/50
0.0008*

9. Control vs. Rinse & Chill
CFU/g

10. Rinse & Chill Technology On-going Protection
Significant on-going protection in further processing of carcasses, such as in ground beef
Inoculated with E. coli 0157:H7;
Vacuum packaged; 40F; 2 trials; triplicate
Ave. (n=6)
Day 1
Log10 CFU/g
Day 17
Day 31
Day 50
Day 70
Day 92 C
4.65
3.81
3.06
2.68
2.77
2.27
R&C
4.76
3.39
2.05
1.81
2.07
0.82
P value
(95% - .05)
0.141
0.005*
0.007*
0.002*
0.0006*
0.021*

11. Vacuum-packaged ground beef; 40 F E. coli 0157:H7 Control vs
Vacuum-packaged ground beef; 40 F E. coli 0157:H7 Control vs. Rinse & Chill
Log10 CFU/g
Day

12. Rinse & Chill Technology Summary
Carcass sponge
Reduction in APC
40%
41.2%
Reduction in coliforms
99.3%
67.8%
Reduction in generic E. coli
83.7%
Ground shoulder/chuck (50/50)
Control – 2.38 log reduction in E. coli 0157:H7 in 91 days
Rinse & Chill – 3.94 log reduction in E. coli 0157:H7 in 91 days