1. Relatieve affiniteit voor de alfa en beta receptor

2. Adapted from Boterberg, 2000 E2E2 

3. GENISTEIN, DAIDZEIN, AND EQUOL AND CELL-CELL ADHESION IN MCF- 7/6 CELLS DMSOEquol Equol + ICI 182,780 Equol + MB2 SLOW AGGREGATION ASSAY

4. FAST AGGREGATION ASSAY

5. Het grote probleem variatie in resultaten van studies bij fyto oestrogenen is de grote variatie in opname van deze stoffen.

6. Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12 th 2009 WORK PACKAGE I O-desmethylangolensinEquol 8-prenylnaringenin DaidzeinIsoxanthohumol Enterodiol (END)Enterolactone (ENL) Secoisolariciresinol  Large interindividual variation

7. Daidzein OHO O OH Dihydrodaidzein (DHD) OHO O O H Equol OHO OH O-desmethylangolensin (O-DMA) OHO O O H H SOY Isoxanthohumol (IX) 8-Prenylnaringenin (8-PN) OMe OH HOP Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12 th 2009

8. MIX Daidzein OHO O OH Equol OHO OH IX OMe 8-PN OH ENL SECO R: H GUT MICROBIOTA Daidzein OHO O OH Equol OHO OH IX OMe 8-PN OH ENL SECO R: H GUT MICROBIOTA

9. Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12 th 2009 WORK PACKAGE I Individual phytoestrogens – SHIME run Individual phytoestrogens – SHIME run

10. Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12 th 2009 WORK PACKAGE I Individual phytoestrogens – SHIME run Individual phytoestrogens – SHIME run Daidzein metabolism (Decroos et al. (2006) J Nutr 136, 946-52) Daidzein metabolism (Decroos et al. (2006) J Nutr 136, 946-52) Weak equol-producing inoculum + EPC4 Weak equol-producing inoculum + EPC4 Standard SHIME feed + 175 µM daidzein aglycon eq. Standard SHIME feed + 175 µM daidzein aglycon eq. IX metabolism (Possemiers et al. (2006) J Nutr 136, 1862-7; Possemiers et al. (2008) J Nutr 138, 1310-6) IX metabolism (Possemiers et al. (2006) J Nutr 136, 1862-7; Possemiers et al. (2008) J Nutr 138, 1310-6) Strong 8-PN-producing inoculum Strong 8-PN-producing inoculum Weak 8-PN-producing inoculum + E. limosum Weak 8-PN-producing inoculum + E. limosum Standard SHIME feed + 70 µM IX Standard SHIME feed + 70 µM IX  Stable EQUOL production in colon transversum  Stable 8-PN production in colon transversum

11. BREAST TISSUE HOMOGENATE

12. MATERIAL & METHODS - BREAST FFQ, BMI, age, antibiotic use,… bloodspot urine Breath methane 1 2 3 45 6 7 8 910R WASHOUT TREATMENT GC-FID AESTHETIC BREAST SURGERY Breast biopsies Enzymatic deconjugation Extraction HPLC-UV-MS Enzymatic deconjugation Extraction HPLC-UV-MS Bio-activity biodistribution concentration molecular form