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Basic Cell Structure
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Cells Basic building blocks of life
Understanding of cell morphology is critical to the study of biotechnology
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Cell Smallest living unit of an organism
Grow, reproduce, use energy, adapt, respond to their environment
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Cell Many cannot be seen with the naked eye
A cell may be an entire organism or it may be one of billions of cells that make up the organism
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Cells Divided and classified in many ways
One common classification method is the presence or absence of a cell necleus
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Nucleus Relatively large structure with a cell Directs cell activities
Some simple single celled organisms lack a nucleus
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Nucleus Prokaryotes – have no nucleus Eukaryotes – have a nucleus
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Prokaryotes Single celled organism Kingdom Monera Lacks a cell nucleus
Internal structure is less organized than other cells
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Prokaryotes Genetic info needed is within the cell
Two most common – blue green bacteria and true bacteria
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Blue green bacteria Have ability to produce their own food like plants
Most bacteria get their food from other sources
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Prokaryotes Less complex than eukaryotes
Still have many of the basic internal characteristics
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Common prokaryote structures
Cytoplasm Chromosomal material Cell membrane and wall Ribosomes Some have flagellum for mobility
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Eukaryotes Organism made up of cells that have a nucleus
May be single celled – Kingdom Protista May have billions of cells like plant and animal kingdoms
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Eukaryotes Single celled eukaryotes include paramecia, euglena, and diatoms
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Eukaryotes Typically are highly organized
Infinite number of shapes and purposes
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Eukaryotes Human body has at least 85 different cell types
All eukaryotes have a number of structures in common
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Common structures Organelles – smaller parts of the cell with specific funtions Cell membrane – acts as a gatekeeper
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Cell membrane Controls what enters and leaves the cell
Plants, algae and bacteria typically have a tough outer structure known as a cell wall
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Cell wall Provides additional structure and protects the cell from pressure caused by movement of water Animal cells do not have walls
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Cytoplasm Organelles are surrounded by a jelly like substance
Primary component is water
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Nucleus Largest and most identifiable part of the cell
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Other organelles Ribosomes Mitochondria Golgi bodies
Endoplasmic reticulum
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Other organelles Lysosomes Vacuoles Chloroplasts in plants
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Nucleus Brain of the cell
Site of much of the manipulation done in biotechnology
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Nucleus Controls activity of the cell Round or oval structure
Typically found in the middle of the cell Appears darker than surrounding material
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Nucleus Surrounded by a membrane that controls passage of materials in and out of the area
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Nucleus Contains DNA DNA is located on chromosomes
Most organisms are diploid – have two chromosomes for each trait
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Diploid Humans – 23 pair Goldfish – 90 chrms, 45 pair
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Cytoplasmic Organelles
Organelles are required for proper cell function Ribosome – make proteins for cellular use and communication May be attached to endoplasmic reticulum
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Endoplasmic reticulum
Layered membraneous Make and transport proteins
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Vacuole Large organelle, without specific shape
Store waste or raw materials used in synthesis of proteins
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Golgi Bodies Golgi apparatus Similar to endo. Ret.
Look like a thick elastic band that has been folded several times
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Golgi Bodies Adds modifications to unfinished proteins Makes lysosomes
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Lysosome Small round Store enzymes that break down food into chemical compounds Destroy cell organelles and the cell itself
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Mitochondria Energy factories Rod shaped
Change food into molecules that can be used for energy
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Mitochondria Contain DNA Can replicate themselves
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Chlorplast Found in plants and some other organisms
Contain chlorophyll
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Cellular processes Respiration Osmosis Diffusion Photosynthesis
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Respiration Cells break down carbohydrates and other molecules to produce energy Oxidation of glucose is one of most common forms
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Respiration Breaking down of sugar into water, carbon dioxide, and energy Energy is used to do the work of the cell
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Osmosis & Diffusion Transport of raw materials, wastes, and synthesized materials out of the cell
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Diffusion Movement of molecules, especially gases and liquids from areas of high concentration to areas of low concentration
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Osmosis Specific type of diffusion
Movement of water through a membrane from areas of high concentration to areas of low concentration
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Equilibrium Diffusion and osmosis happen to enable a cell to reach equilibrium
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Cellular reproduction
Three ways Fission Mitosis Meiosis
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Fission Prokaryotes Cell grows larger and makes a second copy of its DNA At some point the cell membrane divides the cell by the growth of a transverse septum
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Fission Two new cells are formed as the division grows inward from either side of the cell Two cells now called “daughter cells” Sometimes known as “binary fission”
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Mitosis Eukaryotes Requires 6 stages
Interphase, prophase, metaphase, anaphase, telophase, cytokinesis
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Mitosis Creates cells that are identical to the original cell.
Have the entire compliment of chromosomes existing in pairs - diploid
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Meiosis Division of sex cells
Stages of Meiosis I: Interphase I, prophase I, metaphase I, anaphase I, telophase I, cytokinesis I
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Meiosis Meiosis II Interphase II, prophase II, metaphase II, anaphase II, telophase II, cytokinesis II
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Meiosis Each cell receives one chromosome from each pair randomly
Resulting cells have half the normal number of Chromosomes - haploid
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