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Jiangsu Institute of Nuclear Medicine Contact:

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1 Jiangsu Institute of Nuclear Medicine Contact: zhangli@jsinm.org
10th AOTA Congress BALI, INDONESIA, 2012 Curcumin Induces both Apoptosis and Autophagy in Human Papillary Thyroid Carcinoma BCPAP Cells Zhang Li Jiangsu Institute of Nuclear Medicine Wuxi, Jiangsu, China Contact:

2 Curcumin ? An acitve polyphenol derived from the plant turmeric
low toxicity anti-inflammatory antioxidant antiangiogenic antitumour Curcumin Thyroid cancer ? Chemical structure of curcumin

3 Apoptosis and Autophagy
Apoptosis –type I programmed cell death Autophagy- type II programmed cell death - a self-degradative process - cope with stresses - required in apoptotic-deficient cells Three types of autophgay: Microautophagy Chaperone-mediated autophagy Macroautopahgy

4 Part I Crucumin suppresses BCPAP cells growth

5 Effects of curcumin on cell viability

6 Effects of curcumin on cell morphology

7 PI/Hoechst staining A B

8 Part II Curcumin induces apoptosis in BCPAP cells

9 Effects of curcumin on pro-apoptotic and anti-apoptotic proteins
B

10 Effects of curcumin on loss of mitochondrial membrane potential (MMP)

11 Caspase-dependent apoptosis in curcumin-treated cells

12 Part III Curcumin induces autophagy in BCPAP cells

13 Induction of autophagy in curcumin-treated cells
B The FASEB JournalJune 2003vol. 17 no. 9 

14 Induction of autophagy in curcumin-treated cells
RFP-LC3 Curcumin (mM) 50 C

15 Effects of curcumin on the phosphorylation of AKT pathway
B

16 Effects of curcumin on the phosphorylation of MAP kinases

17 Curcumin selectively induces autopagy in tumor cells
B

18 Curcumin seletively induces cancer cell death
B

19 Dynamic distribution of curcumin in BCPAP cells
Enlarged 6 h 12 h 24 h

20 Effects of curcumin on DNA conformation
B

21 Conclusions Curcumin induces cytotoxicity of BCPAP cells.
Induction of  apoptosis  and autophagy as the likely underlying mechanism that is responsible for the cytotoxicity of curcumin . Curcumin inhibited AKT pathway and activated mitogen-activated protein kinase (MAPK) signaling thereby inducing autophagy.

22 Thank you for your attention!
Acknowledgements Jiangsu Institute of Nuclear Medicine Grants Bao Jiandong Yu Huixin Zhang Chiyu Wu Jing Zheng Jie Sun Wei China Medical University Guan Haixia Nangjing University Sun Yang National Natural Science Foundation of China (No ) Natural Science Foundation of Jiangsu Province (No. BK ) Science and Research Foundation of Health Bureau of Jiangsu Province (No. H ) Thank you for your attention!

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