1. Hamilton Venus Software Intelligent Liquid Sensing
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2. Intelligent Liquid Sensing
1. Capacitive Liquid Level Detection (cLLD)
2. Pressure Liquid Level Detection (pLLD)
3. Anti Droplet Control (ADC)
4. Capacitance Clot Detection
5. Monitored Air Displacement (MAD)
6. Total Aspiration and Dispense Monitoring (TADM)

3. Intelligent Liquid Sensing
Capacitance Liquid Level Detection
Conditions:
Black tips or needles
Conductive liquids only
Clean or used tips
All channels
96 head - A1 and H12 only
384 Head – A5 and P24 only
Aspirate and or dispense steps
More Details

4. Intelligent Liquid Sensing
cLLD in detail
Select cLLD
Select cLLD
Set submerge depth (mm down from detected liquid)
Set submerge depth (mm down from detected liquid)
Click Customize for more
Click Customize for more

5. Intelligent Liquid Sensing
cLLD in detail
Select cLLD
Set submerge depth (mm down from detected liquid)
Click Customize for more
Set sensitivity
Set sensitivity

6. Intelligent Liquid Sensing
1. Capacitive Liquid Level Detection (cLLD)
2. Pressure Liquid Level Detection (pLLD)
3. Anti Droplet Control (ADC)
4. Capacitance Clot Detection
5. Monitored Air Displacement (MAD)
6. Total Aspiration and Dispense Monitoring (TADM)

7. Intelligent Liquid Sensing
Pressure Liquid Level Detection
Conditions:
Black / clear tips
Any liquid
Clean tips, aspirate only
All channels
More Details

8. Intelligent Liquid Sensing
pLLD in detail
Select pLLD
Select pLLD
Set submerge depth (mm down from detected liquid))
Set submerge depth (mm down from detected liquid))
Click Customize for more
Click Customize for more

9. Intelligent Liquid Sensing
pLLD in detail
Select pLLD
Set submerge depth (mm down from detected liquid))
Click Customize for more
Set sensitivity
Set sensitivity

10. Intelligent Liquid Sensing
When to use cLLD & pLLD
Bubbles on liquid surface
Use pLLD to find the liquid surface
cLLD could be triggered by the bubbles
Aqueous layer under organic layer
Use pLLD to find the non-conductive organic layer
Use cLLD to find the conductive aqueous layer

11. Intelligent Liquid Sensing
1. Capacitive Liquid Level Detection (cLLD)
2. Pressure Liquid Level Detection (pLLD)
3. Anti Droplet Control (ADC)
4. Capacitance Clot Detection
5. Monitored Air Displacement (MAD)
6. Total Aspiration and Dispense Monitoring (TADM)

12. Intelligent Liquid Sensing
Anti-Droplet Control
Without ADC – high vapour pressure of volatile liquid results in a pressure increase in the tip forcing the liquid out.
With ADC – the pressure increase inside the channel is measured and the piston moved to compensate.
Plunger position

13. Intelligent Liquid Sensing
Anti-Droplet Control
Prevent droplets on tips when pipetting highly volatile liquids This is a standard feature in VENUS
Conditions:
Black / clear tips or needles
Channels only
Pre-wetting tips improves performance
More Details

14. Intelligent Liquid Sensing
ADC in detail
Add HSLML_STARLib to method
Turn ADC on immediately after the aspirate step
Turn ADC on immediately after the aspirate step
Turn ADC off immediately after the dispense step
Turn ADC off immediately after the dispense step
Turn ADC off in the OnAbort submethod too
Turn ADC off in the OnAbort submethod too

15. Intelligent Liquid Sensing
ADC in detail
If the initial pressure rise is very high, ADC will error. Pre-wetting the tip allows the pressure to reach equilibrium before the aspirate
Click customise to change advanced settings
Click customise to change advanced settings
Pre-wet the tip using a mix on the aspirate step
Click Error Settings to set reaction to ADC error

16. Intelligent Liquid Sensing
ADC in detail
If the initial pressure rise is very high, ADC will error. Pre-wet the tip to allow the pressure to reach equilibrium before the aspirate
Click customise to change advanced settings
Pre-wet the tip using a mix on the aspirate step
Pre-wet the tip using a mix on the aspirate step
Click Error Settings to set reaction to ADC error

17. Intelligent Liquid Sensing
ADC in detail
If the initial pressure rise is very high, ADC will error. Pre-wet the tip to allow the pressure to reach equilibrium before the aspirate
Click customise to change advanced settings
Pre-wet the tip using a mix on the aspirate step
Click Error Settings to set reaction to ADC error
Click Error Settings to set reaction to ADC error

18. Intelligent Liquid Sensing
1. Capacitive Liquid Level Detection (cLLD)
2. Pressure Liquid Level Detection (pLLD)
3. Anti Droplet Control (ADC)
4. Capacitance Clot Detection
5. Monitored Air Displacement (MAD)
6. Total Aspiration and Dispense Monitoring (TADM)

19. Intelligent Liquid Sensing
Capacitance Clot Detection
The conductive signal after aspiration is measured.
gap between tip and liquid - the capacitance signal will vanish.
clot - it bridges the gap and the signal remains, resulting in an error message
Conditions:
Black tips or needles
Conductive liquids only
Clean or used tips
All channels
More Details

20. Intelligent Liquid Sensing
Capacitance Clot Detection in detail
Add HSLML_STARLib to method
Turn clot detection monitoring on
Turn clot detection monitoring on
Turn clot detection monitoring off after aspirate
Turn clot detection monitoring off after aspirate
Turn clot detection monitoring off in the OnAbort submethod too
Turn clot detection monitoring off in the OnAbort submethod too

21. Intelligent Liquid Sensing
Capacitance clot detection in detail
Works only with cLLD
Works only with cLLD
Click Error Settings to set reaction on error
Click Error Settings to set reaction on error

22. Intelligent Liquid Sensing
Capacitance Clot Detection in detail
The clot retract height is set in the liquid class
The clot retract height is set in the liquid class

23. Intelligent Liquid Sensing
1. Capacitive Liquid Level Detection (cLLD)
2. Pressure Liquid Level Detection (pLLD)
3. Anti Droplet Control (ADC)
4. Capacitance Clot Detection
5. Monitored Air Displacement (MAD)
6. Total Aspiration and Dispense Monitoring (TADM)

24. Intelligent Liquid Sensing
Monitored Air Displacement
Empty tip
Tip in liquid start aspirating
Pressure
Tip filled with correct volume of liquid
Tip filling with liquid under vacuum
Plunger stops moving, tip continues to fill
Correct Aspiration
Pressure Threshold
Tip blocked
Tip Blocked
Time

25. Intelligent Liquid Sensing
Monitored Air Displacement
Conditions:
Black / clear tips
Clean tips, aspirate only
All channels
Lower limit varies but >10µl
MAD has only been optimised for aqueous liquids
MAD can be switched on for individual aspiration steps in a method
The pressure threshold can be varied to detect whether each pipetting step was successful
More Details

26. Intelligent Liquid Sensing
MAD in detail
Add HSLML_STARLib to method
Set pressure threshold
Set pressure threshold
Turn aspiration monitoring on
Turn aspiration monitoring on
Turn aspiration monitoring off after aspirate
Turn aspiration monitoring off after aspirate
Turn aspiration monitoring off in the OnAbort submethod too
Turn aspiration monitoring off in the OnAbort submethod too

27. Intelligent Liquid Sensing
MAD in detail
Works with or without pLLD
Works with or without pLLD
Click Error Settings to set reaction if pressure threshold is crossed
Click Error Settings to set reaction if pressure threshold is crossed

28. Intelligent Liquid Sensing
1. Capacitive Liquid Level Detection (cLLD)
2. Pressure Liquid Level Detection (pLLD)
3. Anti Droplet Control (ADC)
4. Capacitance Clot Detection
5. Monitored Air Displacement (MAD)
6. Total Aspiration and Dispense Monitoring (TADM)

29. Intelligent Liquid Sensing
Total Aspiration and Dispense Monitoring
TADM increases the safety and robustness of pipetting processes by recording and monitoring pressure throughout the pipetting cycle. It’s main use in in clinical settings where a permanent record showing correct pipetting of every sample is needed.
Conditions:
Black / clear tips
Clean tips
All channels
96 TADM head
Requires additional TADM software

30. Intelligent Liquid Sensing
Total Aspiration and Dispense Monitoring
Aspirate
Dispense
Various pipetting errors change the pressure curves in interpretable ways

31. Intelligent Liquid Sensing
Total Aspiration and Dispense Monitoring
Aspirate
Dispense
The user sets both TADM tolerance bands and the required response if they are crossed
Bands are set , using test data, for every liquid, tip type and volume used