1. The identification proteins using mass spectrometry
“mass-mapping / peptide mass-fingerprinting”
Dr Kevin Mills
Institute of Child Health, UCL, London

2. protein The identification proteins using mass spectrometry
- amino acid
Dr Kevin Mills
Institute of Child Health, UCL, London

3. protein The identification proteins using mass spectrometry - arginine
- lysine
Dr Kevin Mills
Institute of Child Health, UCL, London

4. The identification proteins using mass spectrometry
Recognises and cleaves the
protein at the carboxylic acid
of arginine and lysine amino acids
Trypsin
- arginine
- lysine
Dr Kevin Mills
Institute of Child Health, UCL, London

5. The identification proteins using mass spectrometry
- arginine
- lysine
Dr Kevin Mills
Institute of Child Health, UCL, London

6. The identification proteins using mass spectrometry
- arginine
- lysine
Dr Kevin Mills
Institute of Child Health, UCL, London

7. The identification proteins using mass spectrometry
Dr Kevin Mills
Institute of Child Health, UCL, London

8. The identification proteins using mass spectrometry
Dr Kevin Mills
Institute of Child Health, UCL, London

9. The identification proteins using mass spectrometry
Tryptic digestion of the protein provides peptide
fragments of varying length and therefore mass
Dr Kevin Mills
Institute of Child Health, UCL, London

10. The identification proteins using mass spectrometry
Mass spectrometer
Increasing Mass 
(longer the peptide the heavier its mass)
Tryptic digestion of the protein provides peptide
fragments of varying length and therefore mass
Dr Kevin Mills
Institute of Child Health, UCL, London

11. The identification proteins using mass spectrometry
Mass spectrometer
Increasing Mass 
(longer the peptide the heavier its mass)
Tryptic digestion of the protein provides peptide
fragments of varying length and therefore mass
Dr Kevin Mills
Institute of Child Health, UCL, London

12. The identification proteins using mass spectrometry
Mass spectrometer
Increasing Mass 
(longer the peptide the heavier its mass)
Tryptic digestion of the protein provides peptide
fragments of varying length and therefore mass
Dr Kevin Mills
Institute of Child Health, UCL, London

13. The identification proteins using mass spectrometry
Mass spectrometer
Increasing Mass 
(longer the peptide the heavier its mass)
Tryptic digestion of the protein provides peptide
fragments of varying length and therefore mass
Dr Kevin Mills
Institute of Child Health, UCL, London

14. The identification proteins using mass spectrometry
Mass spectrometer
Increasing Mass 
(longer the peptide the heavier its mass)
Tryptic digestion of the protein provides peptide
fragments of varying length and therefore mass
Dr Kevin Mills
Institute of Child Health, UCL, London

15. The identification proteins using mass spectrometry
Mass spectrometer
Increasing Mass 
(longer the peptide the heavier its mass)
Tryptic digestion of the protein provides peptide
fragments of varying length and therefore mass
Dr Kevin Mills
Institute of Child Health, UCL, London

16. The identification proteins using mass spectrometry
Mass spectrometer
Increasing Mass 
(longer the peptide the heavier its mass)
Tryptic digestion of the protein provides peptide
fragments of varying length and therefore mass
Dr Kevin Mills
Institute of Child Health, UCL, London

17. The identification proteins using mass spectrometry
Mass spectrometer
Increasing Mass 
(longer the peptide the heavier its mass)
Tryptic digestion of the protein provides peptide
fragments of varying length and therefore mass
Dr Kevin Mills
Institute of Child Health, UCL, London

18. The identification proteins using mass spectrometry
Mass spectrometer
Increasing Mass 
(longer the peptide the heavier its mass)
Tryptic digestion of the protein provides peptide
fragments of varying length and therefore mass
Dr Kevin Mills
Institute of Child Health, UCL, London

19. The identification proteins using mass spectrometry
Increasing peptide length results in increasing mass of the peptide
100
1283.6
2172.2
1531.7 %
1586.8
1478.8
1690.9
1125.6
2212.1
1195.5
1430.7
1840.1
1953.8
m/z
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
2500
Dr Kevin Mills
Institute of Child Health, UCL, London

20. The identification proteins using mass spectrometry
Trypsin fragment
Dr Kevin Mills
Institute of Child Health, UCL, London

21. Identification of Proteins by Peptide Mass Mapping
1antitrypsin
deficiency
Comparison of
peptide masses
with in silico
digestion in data
base to obtain
“match”
control
plasma
In-gel
Tryptic
digestion
3D Model of a1-antitrypsin
Dr Kevin Mills
Institute of Child Health, UCL, London

22. Content on this website is the copyright of the Great Ormond Street Hospital for Children and the Institute of Child Health unless otherwise stated. Where copyright applies, visitors can download material for private research, study or in-house use only. Visitors must not copy, distribute, or publish any material from this website. Any other use of copyright material requires the formal permission of the Great Ormond Street Hospital for Children and the Institute of Child Health. Information specifically for patients and families has been reviewed for accuracy, but we hold no responsibility for alterations made thereafter without our consent.
The content of this presentation is the copyright of Dr Kevin Mills, UCL Institute of Child Health. Visitors must not copy, distribute, or publish any material from this presentation. It is strictly forbidden to alter this presentation in any way. Any other use of this presentation requires formal permission from Dr Mills.
(c) Dr Kevin Mills, UCL Institute of Child Health, London, 2006.