1. 1 E-Seminar Protocols Press *6 to mute (listen) Press #6 to un-mute (ask a question) As a courtesy to others, please mute your phone line while listening

2. 2 One-Minute NMR 2007 demo tour University of Buffalo: Buffalo, NY; January 8 TriMR Regional Meeting: Research Triangle Pk, NC; Feb 15-16 Southwest Regional Demonstration, San Diego, CA; Mar 27 (anticip) ENC: Daytona Beach, FL; April 22 – 26 (anticip) Schedule a live demo in your area! Call Protasis for details. 6 March, 2007 Presented by Tim Peck

3. 3 One-Minute NMR Winter 2006-07

4. 4 Metabonomics Research Day University of Buffalo Amherst January 8, 2007 Natural Sciences Complex Center for Tomorrow

5. 5 Metabonomics Research Day University of Buffalo Amherst Morning Session Metabonomics and Top-Down Systems Biology: From Personalized Healthcare to Molecular Epidemiology Plenary Speaker: Professor Jeremy Nicholson 1H-NMR Metabonomics for Detecting Epithelial Ovarian Cancer Dr. Kunle Odunsi Dept of Gynecologic Oncology RPCI Prof. Gail Willsky Dept of Biochemistry Univ. Buffalo Effects of Ligand and Ability to Lower Blood Glucose on Global Gene Expression in STZ- Diabetic Rats Treated with Oxovanadium Compounds Prof. Frank Schroeder Cornell University and Harvard Medical School The virtue of the impure: chemical biology revealed by direct NMR

6. 6 Metabonomics Research Day University of Buffalo Amherst Afternoon Roundtable Session 1: Scientific Infrastructure for Metabonomics – Instrumentation, Software and Databases Dinesh Sukumaran, Ph.D. Director, Magnetic Resonance Center Department of Chemistry, U. Buffalo Session 2: Synergy of NMR Spectroscopy and Mass Spectrometry in Metabonomics Prof. Thomas Szyperski Dept. of Chemistry and Biochemistry U. Buffalo Session 3: Metabonomics – Related Research Prof. George DeTitta Dept. Structural Biology U. Buffalo Session 4: Metabonomics in an ‘ohmics’ world Prof. Jeremy Nicholson Dept. of Biological Chemistry Imperial College

7. 7 TriMR Regional Meeting Research Triangle Park Feb. 15, 2007 Hosted at GlaxoSmithKline Vs.

8. 8 TriMR Regional Meeting Research Triangle Park Feb. 15, 2007

9. 9 One-Minute NMR: Integrated System Approach 6- or 12-Tray Capacity for Vials or Trays Multi-Solvent Wash Station Sample Return Accident-Free Syringe and Needle Web-based Software Well Plate or Vial Sample Format CapNMR Probe 5- or 10-µL Discovery Tower On-Board Instrument Server Microliter precision 0.1 mm xyz positioning Well Plate or Vial Tray

10. 10 Automation: Accident-Free Syringe and Needle Carriage comes to rest before needle is lowered

11. 11 Automation: Solvent Conditioner Solvent Bottles Under Positive Pressure Solvent Conditioner Vacuum Pump Result: Dry, Degassed, Protected Deuterated Solvent

12. 12 Integrated Approach

13. 13 MicroFlow NMR Literature www.microNMR.com

14. 14 Your MicroFlow NMR Product Source www.protasis.com