1. Semen Preparation using the Zavos Swim-up Column (ZSC)
Dr. Panayiotis M. Zavos, Ed.S., Ph.D.
President and CEO
Zavos Diagnostic Laboratories, Inc.
Lexington, Kentucky
USA
“We did all this for you”
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2. Zavos Swim-up Column
The ZSC-II Kit includes ZSC-II, plastic pipette, and 1mL syringe with needle.
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3. Additional materials: SpermPrep Media LabTop incubator IUI Catheter
Zavos Swim-up Column
Additional materials:
SpermPrep Media
LabTop incubator
IUI Catheter
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4. Remove ZSC from the pack and using sterile techniques, uncap the ZSC.
Zavos Swim-up Column
Remove ZSC from the pack and using sterile techniques, uncap the ZSC.
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5. Zavos Swim-up Column
Add 2mL of semen directly into the bottom cone of the ZSC using the plastic pipette.
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6. Zavos Swim-up Column
If the volume of semen is less than 2mL and does not reach the top of the cone, then gently add SpermPrep Media up to the top of the cone to make up the difference.
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7. Zavos Swim-up Column
Using the syringe with needle, add 0.7mL of SpermPrep Media on the outside of the cone up to the top of the cone until the media touches and covers the semen.
“This allows the healthy sperm to swim out of the semen and into the media.”
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8. Place the ZSC in the LabTop Incubator which is set at 35°C
Zavos Swim-up Column
Place the ZSC in the LabTop Incubator which is set at 35°C
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9. Zavos Swim-up Column
Leave the ZSC in the LabTop Incubator for minutes.
“This allows adequate numbers of high quality sperm to swim into the over layered media.”
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10. Zavos Swim-up Column
Aspirate all the media, which should contain all the motile and healthy sperm, from the outside of the cone.
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11. Zavos Swim-up Column
Simply, attach the syringe to the catheter of your choice (TomCat shown) and perform the Intra Uterine Insemination (IUI).
“It is that simple!”
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12. Zavos Swim-up Column
To order your ZSC Kits, LabTop incubator, SpermPrep Media, and IUI Catheters, please call us at or
You may also us you order at
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