1. Aulami "Biokimia" Presentation 8 Aulanni’am Biochemistry Laboratory Chemistry Departement Brawijaya University Basic Structure of Nucleic Acids

2. Aulami "Biokimia" Presentation 8 Basic Structure of Nucleic Acids Monophosphate Diphosphate Triphosphate Adenine Guanine Thymine Cytosine Uracil Nucleo s ide (Adenosine) Nucleo t ide (Adenosine monophosphate, AMP) Purine Pyrimidine Ribose, Deoxyribose 1’ 2’3’ 4’ 5’

3. Aulami "Biokimia" Presentation 8 Nucleotides Linked by Phosphodiester Bond OH PO 4 2- Phosphodiester bond O-P=O - O O 5’ 3’ PO 4 2- OH 3’ 5’ P R P R P R P R P R P R 1 2 3’ 5’ 1 2 3 4 5 6 A kind of phospho-polysaccharides

4. Aulami "Biokimia" Presentation 8 The Notation for Nucleic Acids P R B 3’ 2’ 5’ 1’ ATCGATCG P OH 5’3’ 5’ pApTpCpGpApTpCpG -OH 3’ 5’ p ATCGATCG - OH 3’ ATCGATCG

5. Aulami "Biokimia" Presentation 8 The Two Chains of DNA Are Antiparallel 5’ pCpGpApTpCpGpApT -OH 3’ 5’ pApTpCpGpApTpCpG -OH 3’ 5’3’ 5’

6. Aulami "Biokimia" Presentation 8 Large groove Small groove 1 Twist = 10.5 bp 1 2 3 4 5 6 7 8 9 10 36 Å Characteristics of Double Helix 3’ 5’ 3’

7. Aulami "Biokimia" Presentation 8