1. Microbiology 156 http://chsweb.lr.k12.nj.us/psidelsky
Mrs. Sidelsky

2. Class One Please sign safety forms
Make sure you understand the safety rules
Be able to identify safety equipment in the room

3. Dress for Laboratory

4. Laboratory Laboratory coat is required Hair should be tied back
No open shoes( sandals)
No Gum, food, beverages
Keep personal belongings on shelves at the entrance to the laboratory
Prepare for each class by reading laboratory experiment(s) for class

5. Journal For the second class you will need a Journal
The Journal can be a marble notebook or spiral bound notebook. It will consist of laboratory experiments for each class.
Directions for your laboratory manual will be provided during the second class

6. Compound Light Microscope

7. Introducing your microscope
Always clean lenses before starting
Always with both hands supporting base
Store with arm facing the door. Cover
Cleaning lenses before and after using
Focus with scanning and low before proceeding to high and oil
Learn the most effective ways to regulate and manage light

8. Objective Lenses Objective Lens Mag Total Mag Color of Lens Use
Scaniing 5 50
Red
Observe the entire slide. Focus with coarse adjustment
Low 10
100
Yellow
Tweak focus with coarse. Make sure the image is clear and centered
High 40
400
Blue
Focus only with fine adjustment
Oil
1000
White
Apply oil and then turn high power objective lens and focus with fine adj

9. Microscope terms Microscope terms Magnification Total magnification
Resolution
Depth of field
Size of field
Working distance
Parfocal
Parcentric

10. Microscope and Slides Three Threads
Place slide on mechanical stage.  Make sure that stage clips are holding slide securely. 
Use the scanning lens( red) to find the point at which the threads cross.
Focus the microscope .  Using the coarse adjustment
Observe and draw
Change to low power. Turn the revolving nose piece so that the yellow lens is in place. Observe and draw

11. Observations What do you notice about the size of field?????
What do you notice about your ability to focus.
Focus up and down very carefully and slowly with the fine adjustment
What occurs? Why

12. High Power
If your focus is sharp and clear, turn the revolving nosepiece to blue
Use the fine adjustment, focus carefully – you are very close to the slide.
Observe and draw

13. Bacterial Slide Take a slide of a bacillus from the supply area
Place it on the Stage
Repeat the procedure, for focusing
Adjust the light and draw
Observe and draw.

14. Bacterial shapes

15. Bacterial Shapes and Arrangement
Streptococci

16. Rods- Bacilli – Stained with Gram Stain