1. 0 Microbial Gene Research & Resources Facility (MGRRF) Institute for Molecular & Cellular Therapies (IMCT) Biomolecular & Biomedical Sciences (BBS) New Lorne Bore (RN 17263). Depth 1613 m, Temp: 89 o C, pH 8.5); Flow rate: 7.56 L/sec; Chemistry: typical Na-HCO 3 -Cl Brisbane Sampling at bore source Sampling below bore source

2. 1 Microbial Gene Research & Resources Facility (MGRRF) Institute for Molecular & Cellular Therapies (IMCT) Biomolecular & Biomedical Sciences (BBS) The New Lorne Bore (RN 17263) runoff channel Temperature specific microbial colonisation occurs in the New Lorne bore run off channel in a temp gradient (88 o C to ambient) Sampling microbial mats

3. 2 Microbial Gene Research & Resources Facility (MGRRF) Institute for Molecular & Cellular Therapies (IMCT) Biomolecular & Biomedical Sciences (BBS) Grey Filaments at 75ºC Red Filaments at 66ºC Brown Filaments at 52ºC Green Filaments at 57ºC 4 spatially distinct colored microbial mats colonise New Lorne bore runoff channel at different temperatures

4. 5 Samples (4 mats + source sample) HMW DNA extracted 16S rRNA genes cloned 400 clones studied to determine phylogroups Representative of each phylogroup sequenced Phylogenetic Reconstruction

5. D ictyoglomus Thermodesulfobacteria Bacteroides Thermomicrobia Aquifex Chrysiogenetes Thermotoga   T hermales Firmicutes Chlamydia Deferribacter Nitrospir a  Cyanobacteria Actinobacteria V errucomicrobia Acidobacteria Planctomycetes Fibrobacter   Spirochetes Fusiforms Proteobacteria Microbial diversity of the New Lorne bore & the 4 runoff channel microbial mats (10 / 21 kingdoms) 1 2 3 4 5 6 7 8 9 10

6. 5 Microbial Gene Research & Resources Facility (MGRRF) Institute for Molecular & Cellular Therapies (IMCT) Biomolecular & Biomedical Sciences (BBS) The microbial diversity profile of the 4 microbial mats

7. Quantitative diversity based on clones (in %)