1. Career Seminar Series IVF Embryologist Dr Gayle M. Jones Senior Research Fellow Monash Immunology & Stem Cell Laboratories

2. Tertiary Training B.Sc.(HONS) University of Melbourne Ph.D. Department of Pathology, University of Melbourne Certificate Recombinant DNA Techniques – Micromon, Monash University

3. Career Path NH&MRC Senior Technical Officer – Royal Women’s Hospital NH&MRC Research Officer – Royal Women’s Hospital IVF Embryologist – Royal Women’s Hospital Visiting Research Fellow – UC Irvine, California Director of Embryology – Melbourne IVF Research Fellow – Monash Institute of Reproduction & Development Senior Research Fellow – Monash Institute of Reproduction & Development Senior Research Fellow – Monash Immunology & Stem Cell Laboratories

4. Career Opportunities for Scientists in Assisted Reproduction Clinical Embryologist/Preimplantation Geneticist Laboratory Scientist – Pathology Andrology Endocrinology Research Scientist Human Animal species

5. Day 0 – Oocyte Retrieval

6. Day 0 - Insemination

7. Intra cytoplasmic sperm injection - ICSI

8. Day 1 – Fertilization check

9. Embryo Culture – Day2-Day5/6

10. Embryo Transfer

11. EMBRYO BIOPSY 1st PB 2nd PB ICSI 1-2 / 8 cells Trophectoderm  20 cells Day 0 Day 1 Day 3 Day 5

12. Hole in the zona pellucida by non-contact laser Insertion of glass micropipette Removal of a single blastomere for genetic analysis Embryo Biopsy – Day 3 Embryo

13. XXXYXXX Fluorescence In Situ Hybridization XX XY XXX XO Monosomy 21 Trisomy 21

14. Results of Clinical PCR for Cystic Fibrosis – Day 3 Embryos Father (carrier) Embryo (carrier) Embryo (affected) Embryo (unaffected) Mother (carrier) Delta F508Normal