3. Visiting IFE Wednesday 21th Mars?

6. Optical microscopy MENA3100,OBK, 31.01.12

7. Light microscope Lysmikroskop We want to see more details Magnification (forstørrelse) Resolution (oppløsningsevne) Contrast Optical microscope Optisk mikroskop

8. Simple microscope Lupe, forstørrelsesglass

9. The simple microscope Antony van Leeuwenhoek (1632−1723)

10. The compound microscope ca. 1595 Hans Janssen Robert Hooke (1635−1703) Micrographia, 1664 Det sammensatte mikroskop

11. Don’t get too hang up in the ray tracing

12. Stereo microscope Stereomikroskop

13. Compound microscope for transmitted light Mikroskop for gjennomfallende lys

14. Inverted compound microscope for reflected light Mikroskop for reflektert lys

17. www.microscopy-analysis.com/files/ jwiley_microscopy/2006_Sept_Hammond.pdf

18. Illuminating system Specimen Filament Field diaphragm Aperture diaphragm Condenser lens

19. Illuminating system Specimen Filament Field diaphragm Aperture diaphragm Condenser lens Köhler illumination Uniform specimen illumination Field diaphragm Controll illuminated area Aperture diaphragm Controll angle of the cone of light

20. Imaging system Specimen Objective Tube lens Eyepiece Okular

21. Primary image

22. Refraction

23. Kromatisk abberation

24. Spherical abberation

25. CorrectionsThe objective

26. The limiting factor: Diffraction

27. R = 0,61 /NA Resolution Rayleigh criterion (Raleigh in Brandon and Kaplan)

29. Abbe

30. Numerical aperture (NA) NA = nsin  R = 0,61 /NA Resolution

31. Oil immersion

32. Fouriertransformasjon

33. Condenser system Eyepice Ocular (okular) More is needed than just the objective

35. Optical anisotropy Calcite

36. Amorphous and cubic: Optically isotropic, one refracive index Hexagonal, trigonal, tetragonal: Optically anisotropic, two refractive indices Lower symmetry: Optically anisotropic, three refractive indices

37. Optically active crystals in the microscope

39. Michel-Levy chart

41. Remember that geologists often call their book on microscopy on thin sections for optical crystallography, and microscopy with reflected light is ore microscopy (malmmikroskopi). Some web sites: http://www.olympusmicro.com/primer/ http://www.microscopyu.com/ http://www.micro.magnet.fsu.edu/ http://www.micro.magnet.fsu.edu/primer/pdfs/basicsandbeyond.pdf maintenance: http://www.zeiss.de/C1256D18002CC306/0/1205E8CBD68054EAC125701500404705/$file/50-1-0011_e.pdf