1. Chapter 13
Intracellular Vesicular Traffic
張學偉 助理教授

5. The molecular mechanisms of membrane transport and the maintenance of compartmental diversity

8. GFP-FUSION PROTEINS HAVE REVOLUTIONIZED THE STUDY OF INTRACELLULAR TRANSPORT

9. There are various types of coated vesicles
Mediate transport
from Golgi & from
plasma membrane
Mediate transport
from ER & Golgi
Each type is used for different transport steps in the cell.

11. The assembly of a clathrin coat drives
vesicle formation

12. by charperone
(hsp70)
Major coat protein: clathrin & adaptin
There are at least four types of adaptins,
each specific for a different set of cargo receptor.

13. Both the pinching-off and uncoating of coated vesicles are regulated processes

15. Not all transport vesicles are spherical
 Various size & shape

16. Monomeric GTPase control coat assembly

17. SNARE proteins and targeting GTPases guide membrane transport

19. Trans-SNARE complex

20. Interacting SNAREs need to be pried apart
before they can function again
cytosol
Cycle

21. Rab proteins (monomeric GTPase) help ensure the specificity of vesicle docking

22. Rab facilitate docking of transport vescicles.
Rab &its effector
Structure vary greatly
Function common
(1.concentrate & tether
vesicle near target site
2. Trigger release of
SNARP control protein)
cycle

23. SNARE may mediate membrane fusion
Process for SNARE concentrate in membrane fusion

24. Viral fusion proteins and SNAREs may use similar strategies

26. Transport from the ER through the golgi apparatus

28. ER exit site
50nm vesicle
> 200 mem protein
Cargo Recruitment

29. Only proteins that are properly folded and assembled can leave the ER
Incomplete
ER resident protein

30. Defect in Cl- transport
CF (Cystic fibrosis)
Defect in Cl- transport
This is not because the mutation inactivated the protein,
But because the active protein is discarded before it
reaches the plasma membrane.

31. Transport from the ER to the Golgi apparatus
is mediated by vesicular tubular clusters.
Heterotypic fusion
Homotypic memb fusion is not restricted to form VTC.

32. The structure formed when ER-derived
vesicles fuse with one another are called.

33. ER retrival signal

34. The retrieval pathway to the ER uses sorting signals
Lys-Asp-Glu-Leu (KDEL)
Short retrieval signal
at c-terminal
Resident ER membrane protein
KKXX at c-terminal end  direct interact with COPI coat

36. Many proteins are selectively retained in the compartments in which they function
Aggregation of proteins that function in the same compartment
- called kin recognition

37. The golgi apparatus consists of an ordered series of compartments.

41. Two major classes of N-linked oligosaccharide
complex
High mannose
No new sugar added in Golgi

42. Oligosaccharide processing in ER and Golgi

43. High specific
endoglycosidase
Can distinguish between
these two type

44. Proteoglycans are assembled in the Golgi Apparatus
O-linked glycosylation
Proteoglycans are secreted or anchored to plasma membrane

45. What is the purpose of N-glycosylation?
1. N-linked is prevalent in all eukaryotes, but absent in procaryotes.
2. limited flexibility.
3. Recognition
4. Regulation of development
5. Protective coat unit
6. Cell-cell adhesion

46. N-linked

47. The golgi cisternae are organized as a
series of processing compartments

48. Functional compartmentalization

50. Matrix proteins form a dynamic scaffold that helps organize the apparatus

51. Transport from trans golgi network to lysosomes

53. Lysosome are the principal sites
of intracellular digestion

54. Lysosomes are heterogeneous (morphology)
The diversity reflects the wide range of
digestive function.

55. Plant and fungal vacuoles are remarkably verstile lysosomes

56. Controlling size Vacuole function:
Storage, degrade, cell size, turgr pressure, homostatic device

57. Three pathways to degradation in lysosomes.

58. M6P receptor recognizes lysosomal proteins
in the Trans Golgi network (TGN)

59. The M6P receptor shuttles between specific membranes

62. Transport into the cell from the plasma membrane: endocytosis

80. Transport from the trans golgi network to the cell exterior: exocytosis

93. Chapter 13 practice
The endocytic and biosynthetic-secretory and retrieval pathway.
Utilization of different coats in vesicular traffic.
The role of SNAREs and SNAREs-interacting proteins in vesicle transport .
The traffic role of COPI &COPII coat shuttling between Golgi and ER.
What is KDEL signal and its role in retrieval pathway.
How to distinguish between the high-mannose oligosaccharide and complex oligosaccharide.
Write the full name for Glc NAc, NANA(sialic acid).
The functional compartmentization of Golgi apparatus.
Three pathways to degradation in lysosomes.
How to transport lysosomal enzyme to lysosome from ER.
Possible fates for endocytosed transmembrane receptor proteins.
The receptor-mediated endocytosis of LDL.
Three best-understanding pathways of protein sorting in trans Golgi.