1. Bio 104: Issues in Biotechnology
The Way We Work With Life
Dr. Albert P. Kausch
life edu.us
Lecture 26
Part Ic. Animal and Human Cloning
and Genetic Engineering
Medical Biotechnology
© life_edu
Feb 20a: Agricultural Biochechnology

2. Bio 104: Issues in Biotechnology
The Way We Work With Life
Dr. Albert P. Kausch
Kimberly Nelson
OnCampus Live
BCH 190, MIC 190, AFS 190, NRS 190, PLS 190
OnLine BCH 190
A Sweeping General Survey on Life and Biotechnology
A Public Access College Course
The University of Rhode Island
Issues in Biotechnology:
Biotechnology, Our Society and Our Future
life edu.us
Feb 20a: Agricultural Biochechnology

3. Bio 104: Issues in Biotechnology
The Way We Work With Life
Dr. Albert P. Kausch
life edu.us
BCH 190
Section II.
The Applications of
Biotechnology
A Sweeping General Survey on Life and Biotechnology
The University of Rhode Island
© life_edu
Feb 20a: Agricultural Biochechnology

4. Where is DNA in a cell?
All of the information for each protein in a cell is in it’s DNA
All of the information that codes for a complete organism is in DNA

5. Cloning Animals
Cloning and Genetic Engineering in Animals

6. Genetically Engineered Animals
Cloning and Genetic Engineering in Animals

7. How to Clone

8. Nuclear transfer procedures

9. An Organism can be cloned by transferring it’s DNA to an egg cell
This shows:
All of the information that codes for an organism is in it’s DNA
All of the DNA is the same in every cell
DNA is located in the nucleus of the cell

10. Could we Genetically Engineer Cloned Animals? Controlled expression
Promoter
Coding Sequence
Terminator
Your favorite gene
Vaccines
Antibodies
High Value Proteins
Spider Silk in Goats Milk
Controlled expression
“making protein”
Stop transcription
Message stability

11. Genetically Modified somatic nuclei could be implanted into unfertilized eggs to create a genetically modified clone

12. Transgenics and Cloned Animals
Genetically Modified somatic nuclei could be implanted
into unfertilized eggs to create a genetically modified clone

13. What is cloning?
A process whereby all members are directly descended (asexually) from a single organism by……(various ways)
Demonstrates that all the information required for an organism and its development are in the a single cell

14. Where is DNA in a cell?
All of the information for each protein in a cell is in it’s DNA
All of the information that codes for a complete organism is in DNA

16. Principle All cells are derived from a zygote
All have the same genetic makeup
All have the potential to develop into an individual

22. UCONN Clones

23. FIGURE 5-45 Genetically identical cloned animals
FIGURE 5-45 Genetically identical cloned animals. The cloning of animals can maintain desirable traits from generation to generation.

24. Many animals can now be cloned And Genetically Engineered sheep cattle
porcine
mice
rats
mules
frogs
primates?
And Genetically
Engineered

25. Primates Many animals can now be cloned And Genetically Engineered
2012
And Genetically
Engineered

27. Issues in Biotechnology
The ‘cloning’ of plants was demonstrated by Steward in 1958 at Cornell Univ. showing that plants could be grown back from a single cell. This ability is called “totipotency” and like the more recent examples of animal cloning, demonstrates:
(A) that all biological scientists are unethical and fraudulent
that all of the DNA that codes for an entire organism is
in every cell of that organism
Steward belonged to the group that called
themselves the ‘Raeliens’
(D) the necessity to ban all types cloning
(E) that the theory of evolution must be wrong

28. Could we Genetically Engineer Humans? Controlled expression
Promoter
Coding Sequence
Terminator
Your favorite gene
Insulin
Cancer
Anti-Oncogenes
Parkinson’s
Huntington’s
SCID
Beyond Therapy???
Controlled expression
“making protein”
Stop transcription
Message stability

29. Can humans be cloned and/or genetically engineered?
Cells from an amniocentesis
can be used for DNA sequence analysis. All
of the genes for humans will be known.
Disease genes would be detected.
Cloned “correct” genes could be used for replacement.
Corrected nucleus could be delivered to an unfertilized egg.
Same baby without the defect.

30. Could we Genetically Engineer Humans?

31. The Tay Sachs
Dilemma

32. Do You Think We Should We Genetically Engineer Humans?
(A) yes
(B) no
(C) depends on the circumstance

33. Issues in Biotechnology
I would clone myself if that were possible.
(A) yes
(B) no
(C) undecided

34. The Possibilities of
Human Cloning

35. To Clone or
Not to Clone

36. For more information please contact:
For those who are interested in taking this course for college credit through the University of Rhode Island; 
For more information please contact:

37. Credits Lectures by: Edited by: Video Produced by:
Thank You to The University of Rhode Island
and all of the students of Issues in
Biotechnology over the years
Dr. Albert Kausch
and Kimberly Nelson
Thaddeus Weaver