1. Warm-Up Write a reminder for your project, as well as the upcoming test date – get it stamped! Update your table of contents for today! Complete the “Before” column on the Anticipation Guide. DateSession # ActivityPage # 1/13 & 1/14 6 Anticipation Guide & Background Notes 13 “Milk…How Sweet Is It?” Lab Guide 14

2. Warm-Up/Review: What is a catalyst? What is a genotype? What is coevolution?

3. Lactose Vs. Lactase Notes (write what is in black on the slides) Lactose = a sugar found only in milk Lactase = an enzyme found in the lining of the small intestine that acts as a catalyst in the breakup of lactose into glucose, which can be digested by the body Just FYI… Enzyme = a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction

4. Lactose Vs. Lactase What does lactose intolerance mean? So what do you think lactase persistence means? These are genetic characteristics…so, do you think that it is more likely for people to be lactose intolerant or lactase persistent?

5. The Facts About Lactose Intolerance…Remember, Milk has Sugar, Fats & Proteins: Lactose intolerant people cannot digest the sugar (lactose) in milk. It causes diarrhea and other unpleasant symptoms  As a result, the milk “races through” the digestive system… very little of the other nutrients in the milk (fat & protein) don’t remain in the body long enough to be digested.

6. Milk – How Sweet Is It? Lab Your group will have a sample of the “intestinal fluid” of a patient. You will test the fluid to determine if your patient has the gene for lactase persistence and is able to digest milk. Follow the lab directions and share your data with the class by adding your data to the table on the board. Answer the lab questions on your sheet.

7. Milk – How Sweet Is It? Lab Roles Group Leader – reads all directions and keeps group on task; reinforces the jobs of others Supply Manager/Clean Up – manages supplies Math & Map Master – does calculations & is in charge of recording math & map information (someone with a phone) Discussion Leader – Leads the discussion of the questions to share information with the group and the class if required

8. Milk – How Sweet Is It? Lab Results 2 minutes Samples Glucose levels (mg/dL) Patient #1 (Japan) Patient #2 (Kenya) Patient #3 (China) Patient #4 (Italy) Patient #5 (Papua New Guinea) Patient #6 (Finland) Patient #7 (Saudi Arabia) Patient #8 (Uganda)

9. Global Distribution of the Lactase Persistence Genotype You will analyze the distribution of lactase persistence (ability to digest milk) across a large area of the world. 1. Calculate the frequency of lactase persistence for the 3-4 regions on your strip of paper. 2. For each region, you must put a sticker on the board. If the percentage is less than 40%, place a pink sticker, if the percentage is equal to or greater than 40%, place a green sticker. 3. When you have the percentages calculated, send your Math & Map Master to place the stickers on the correct country on the map on the board. = lactose intolerance= lactase persistence

10. 1. Create a key for the map on your answer sheet. - One color for lactase persistence (ability to digest milk ≥ 40%) - One color for lactose intolerance (ability to digest milk < 40%) 2. Using dots or shading, duplicate the class data on the map on your note sheet.

11. Answer the activity questions below the map on your note sheet Global Distribution of the Lactase Persistence Genotype

12. Got Lactase? The Co-Evolution of Genes & Culture Got Lactase: The Co-Evolution of Genes and Culture http://www.hhmi.org/biointeractive/making-fittest-got-lactase-co-evolution-genes-and-culture After the video, take the quiz again, put your answers in the “after” column. Answer the questions following the quiz

13. Global Distribution of the Lactase Persistence Genotype Answer the reflection questions on the back of the video page of your note sheet.