1. Nutrient Trends in Selected Springs of the Santa Fe River Robbie McKinney Environmental Scientist-Biologist Santa Fe Springs Working Group December 11, 2008

2. Background Water Assessment Regional Network – Established 1989 – water quality status – changing conditions Over 10 years of data collection – Springs – Rivers – Lakes Water Assessment Regional Network – Established 1989 – water quality status – changing conditions Over 10 years of data collection – Springs – Rivers – Lakes

3. SRWMD Spring Sampling Sites Poe Springs – June 1997 * Ginnie Springs – November 1997 Blue Springs (High Springs) – December 1992 * Columbia Springs – May 1998 Treehouse Springs – May 1998 Hornsby Springs – November 1992 * Santa Fe Springs – June 1998 * monitored monthly; included in presentation Poe Springs – June 1997 * Ginnie Springs – November 1997 Blue Springs (High Springs) – December 1992 * Columbia Springs – May 1998 Treehouse Springs – May 1998 Hornsby Springs – November 1992 * Santa Fe Springs – June 1998 * monitored monthly; included in presentation

6. Statistical Analyses Data analysis includes – Date collected – Year collected – Means comparisons Spearman Rho Correlations Split-plot Analysis of Variance (ANOVA)

7. Blue Springs at High Springs Gilchrist County Blue Springs Park Second Magnitude 40 foot diameter vent Max. depth is 24 ft. Approx. 2000 ft. spring run

8. 2007-2008 Nutrient Sample Data **No TP correlation with Date, Year** **Correlation between Date, Year, and NOX**

9. Period of Record NOX Trends r = 0.489 p < 0.001

10. NOX Trends by Year r = 0.488 p < 0.001

11. Poe Springs Alachua County Poe Springs County Park Second Magnitude Approx. 150 ft. pool area Max depth is 18 ft. 60 ft. spring run Alachua County Poe Springs County Park Second Magnitude Approx. 150 ft. pool area Max depth is 18 ft. 60 ft. spring run

12. 2007-2008 Nutrient Sample Data **No TP correlation with Date, Year** **Correlation between Date, Year, and NOX**

13. Period of Record NOX Trends r = -0.690 p < 0.001

14. NOX Trends by Year r = -0.688 p < 0.001

15. Hornsby Springs Alachua County Camp Kulaqua Second Magnitude Pool is approx. 300 ft Max. depth is 20 ft. 35 ft. diameter vents 0.8 mile spring run

16. 2007-2008 Nutrient Sample Data **No TP correlation with Date, Year** **Correlation between Date, Year, and NOX**

17. Period of Record NOX Trends r = -0.499 p < 0.001

18. NOX Trends by Year r = -0.493 p < 0.001

19. Nitrate-Nitrite Nitrogen by Site C A B 0.3451.7180.534 p<0.0001

20. Nitrate-Nitrite Nitrogen by Year No Significant Differences Value Range 0.600 – 1.349 p=0.0857

21. Total Phosphorus by Site A A B 0.1020.0460.099 p<0.0001

22. Total Phosphorus by Year A A B AB A = 0.097 B = 0.066 p=0.0009

23. References Boyd, C. E. 2000. Water quality: an introduction. Kluwer Academic Publishers. Norwell, MA. 330 pp. Hornsby, D., R. Ceryak. 1998. Springs of the Suwannee River basin. Suwannee River Water Management District. Live Oak, FL. 183 pp. Hornsby, D., R. A. Mattson, and T. Mirti. 1998. Surfacewater quality and biological annual report. Suwannee River Water Management District. Live Oak, FL. 148 pp. Hornsby, D., R. A. Mattson, and T. Mirti. 2000. Surfacewater quality and biological annual report. Suwannee River Water Management District. Live Oak, FL. 234 pp. Maceina, M. J., P. W. Bettoli, and D. R. DeVries. 1994. Use of a split-plot analysis of variance design for repeated-measures fishery data. Fisheries 19(3): 14-20. SAS Institute Inc. SAS version 8.2 for Windows. Cary, NC: SAS Institute Inc. 1998-2001. SPSS, Inc. SPSS Statistics 17.0. Chicago, IL: SPSS, Inc. 2008 Boyd, C. E. 2000. Water quality: an introduction. Kluwer Academic Publishers. Norwell, MA. 330 pp. Hornsby, D., R. Ceryak. 1998. Springs of the Suwannee River basin. Suwannee River Water Management District. Live Oak, FL. 183 pp. Hornsby, D., R. A. Mattson, and T. Mirti. 1998. Surfacewater quality and biological annual report. Suwannee River Water Management District. Live Oak, FL. 148 pp. Hornsby, D., R. A. Mattson, and T. Mirti. 2000. Surfacewater quality and biological annual report. Suwannee River Water Management District. Live Oak, FL. 234 pp. Maceina, M. J., P. W. Bettoli, and D. R. DeVries. 1994. Use of a split-plot analysis of variance design for repeated-measures fishery data. Fisheries 19(3): 14-20. SAS Institute Inc. SAS version 8.2 for Windows. Cary, NC: SAS Institute Inc. 1998-2001. SPSS, Inc. SPSS Statistics 17.0. Chicago, IL: SPSS, Inc. 2008

24. Contact information Robbie McKinney, M.S. Environmental Scientist-Biologist Suwannee River Water Management District 9225 County Road 49 Live Oak, FL 32060 386.362.1001 800.226.1066 RLM@SRWMD.ORG