1. Biotest Medizintechnik GmbH
Hemoglobin determination Biotest Hemoglobin System Accuracy and precision
Biotest Medizintechnik GmbH

2. Products Cuvette Measuring device Biotest Hemoglobin
Biotest Hemoglobin Tester

3. Function Cuvette Biotest Hemoglobin Tester
Blood capillary, reagent and measuring cuvette in one.
Biotest Hemoglobin Tester
Photometer with integrated control and digital display

4. Function - Cuvette Chemical reaction Haemolysis by sodium desoxycholat
Sodium nitrite
Sodium azid
Methemo-globin
Free
Haemoglobin
Azid-
Methaemo-globin
Erythrocytes

5. Function - Cuvette
Absorbance curve

6. Measurements

7. Measurements
Linearity and precision, n = 20 measurements by each blood sample.
y = 1,0064x - 0,0773
R² = 0,9992
5,00
10,00
15,00
20,00
5,0 g/dl
10,0 g/dl
15,0 g/dl
20,0 g/dl
Biotest Hemoglobin System, g/dl
Mean value HiCN, i g/dl

8. Measurements

9. Function - Tester Photometer; two wave lengths
Evaluation and calculation
Reading
Evaluation

10. Self calibration 100% green light, 100% red light and dark current
Compared to moment of calibration
If necessary automatic adjustment of LED current
Evaluation

11. Resolution Max deviation = +/- 0,75 % or +/- 30 steps Absorption
256 g/L
0 = 100 % light 470 – 880 nm
12 bits = 4000 ”steps”
Resolution

12. Calibration Absorption 256 g/L
Two points of calibration for linearity; 80 and 220 g/L
Three control levels for linearity 120, 150, 180 g/L
0 = 100 % light 470 – 880 nm
12 bits = 4000 ”steps”

13. Function - Tester Calculation of Hb Extinction Turbidity Turbidity
Wave length [nm]

14. Capillary blood Hb
Time
0 min
1 min
2 min
3 min

15. Results - Introduction
Accuracy
How close to true value is the result
Precision
How close to each other are repeated measurements with the same sample

16. Results - Introduction
Accuracy and precision
High accuracy
Low precision
Low accuracy
High precision
High accuracy
High precision

17. Results - Introduction
Accuracy:
Deviation (mean) in g/L
Preferred; deviation in %
Precision:
Standard deviation SD
Preferred; Coefficient of variation, CV %

18. Results Precision with control blood (n=50) Mean value 12,39 g/dl30
Mean value 12,39 g/dl
SD 0,084 g/dl
CV 0,68 % 25 20
Numbers 15 10 5
12,1
12,2
12,3
12,4
12,5
12,6
12,7
Result [g/dl]

19. Features
Cuvette
Aimed for Hb-measurement with Biotest-Hemoglobin-Tester and compatible instruments
High accuracy and precision
Can be used for venous, capillary and arterial blood
Developed to be price worth due to latest production and control technique

20. Features
Tester
Automatic calibration: no calibration or test cuvette needed
Cuvette holder minimizing the risk of contamination
Automatic monitoring of power supply with fast loading of batteries
(1 hour loading > 40 hours of measuring)
Automatic shut off with latest value saved
Measures only cuvettes with properly haemolysed blood

21. Summary High accuracy and precision Easy and reliable system
Long time use on battery power

22. Results - Introduction