2. Cytokine production of human preimplantation embryos M. H. Özörnek, P. Bielfeld, U. Koldovsky Dept. OB&GYN, Heinrich Heine University, Düsseldorf, Germany

3. IFN gamma and IL-10 levels on day 1 vs. day 2

4. IFN gamma levels before implantation day 2day 3day 4day 5

5. n Human embryo do not produce CSF-1 and LIF, at least 2-5 days after fertilisation.

6. EGF production of human embryo

7. Importants of critical selection of recurrent pregnancy loss patients for successful immunotherapy M. H. Özörnek, B. Mikat-Drodzdynski, U. Koldovsky Dept. OB&GYN, Heinrich Heine University, Düsseldorf, Germany

8. Conditions for Immunotherapy University of Düsseldorf n n Primary recurrent pregnancy loss n n Absence of "classical" abortion reasons n n Absence of an autoimmune pathology n n negativ APCA and EAI tests n n negativ infection parameters (HIV,HBs- Ag,Anti HCV,TPHA,CMV)

9. Immunodiagnostik Antipaternal Cytotoxic Antibody (APCA) [cross- match] A test for prooving the presence of cytotoxic antibodies in patient serum against partner lymphocytes Erythrocyt Antibody Rosette Inhibition (EAI) Test A test for prooving the Fc receptor blocking antibodies

10. Protocol of Immunotherapy

11. Immunotherapy results

12. Conclusion 4 4 If RPL is primary, 4 4 If all losses are in 1. Trimester, 4 4 If there are no autoimmune antibodies, 4 4 If both EAI and APCA tests are negativ Immunization is a realistic therapy!