1. QIAGEN 2009 QIAgility ─ Pure Precision Andrea Tesoriero
Application Specialist
QIAGEN 2009

2. You Have More Important Things To Do….
2007
2009
First we automated manual spin column preps…
Now we are able to automate all manual pipetting in the lab…
Now QIAGEN takes the next step and enables another convenience shift

3. From Manual to Automation
Eliminating tedious pipetting in the lab!
Higher Precision and more time every day for…
Preparing & designing new experiments
Data analysis
Literature search
Writing scientific articles
And much more…

4. Developed For PCR Setup QIAgility Main Advantages
Precision Results achieved exceed the best hand pipetting Versatility Supports all different cycler formats in the market Ease-of-use What-you-see-is-what-you-get software interface Speed Streamlined workflow by

5. High Precision Meets Pipetting Skills Precision Aspects
QIAgility performs all “tricks” an experienced technician uses while pipetting
Dynamic liquid level sensing limiting tip immersion
Tracking the meniscus in each tube to minimize tip retention
Retracting the tip while dispensing
Minimizing Tip/Liquid contact
Slowing down pipetting speed for viscous liquids
Avoiding formation of bubbles in tip
Selecting automatically which tip (50µl or 200µl) to select
maximizing precision
38 samples per day

6. The Hallmark Of QIAgility Is Its Versatility Versatility Aspects
No matter what real-time instrument you use, QIAgility is compatible
The system is pre-configured to work with all PCR tube types and all plate formats, including varieties of 96-well, 384-well and Rotor-Disc
Beside any PCR setup the system can perform
Set-up of quantification standards (dilution series)
Normalization of sample concentration
Sample pooling
Transfer of samples from one tube format to another
Automatic set up for multiple variables
Post PCR setup
38 samples per day

7. Versatility QIAgility supports all real-time PCR formats
96-well and 384-well (Incl. all ABI, Bio-Rad, Stratagene, Eppendorf etc. instruments)
SmartCycler™ Tubes
Roche COBAS™
Rotor-Gene™ 0.2 mL, 0.1 mL and Gene-Disc™ formats
LightCycler™ Capillaries 7

8. Versatility 96 and 384-well support
Compatible with plates, tubes, tube racks, cooling blocks etc 8

9. Versatility Unusual formats are also supported
Including: MALDI plates, ELISA plates, reservoir plates, etc, etc 9

10. The Intuitive Software Is Easy To Use Software Features
Ease of Use
The software mirrors the actual deck setup
Intuitive layout—simple to learn
Calculation of reagent needs according to the number of reactions and replicates specified
Tip re-use options
Import/Export of sample names
Pre- and Post-run reports including worktable setup guide
Virtual mode aids new protocol testing
Wizards for speedy PCR setup and optimization
38 samples per day

11. You Have More Important Things To Do…. QIAgility Means Time Savings
Speed
“If you worked once with the QIAgility, you don’t remember how you have managed without…”
Staff can be attending to other duties while the QIAgility is running
Simple Transfer Actions 1:1
96 samples
17 minutes
6 minutes with tip reuse
Transfer Actions with pre-MM
96 samples PCR set-up
~25 minutes
192 samples PCR set-up
~46 minutes
Pfaffl TA testimonial: the CAS1200, the hectic robot…..
38 samples per day

12. Unrivaled Pipetting Precision RotorGene Probe RT PCR Kit
Data presented in brochure table 1

13. Exceptional Reproducibility and Accurate Results RotorGene Probe PCR – Template dilutions
Precision
QIAgility
Setup Details
Target: IL1 R2
Sample: Hs gDNA
10 twofold dilutions
(30ng – 60pg)
5 replicates
Rotorgene 6000
Data presented in brochure figure 1

14. Automated Workflow for Accurate Results RotorGene Probe RT PCR Kit
Precision
Manual
Setup Details
Target: IL1 2RB1
Sample: Leuko RNA
Standard row in triplicates
5 tenfold dilutions (10 pg – 100 ng)
Rotorgene 6000
QIAgility
Data presented in brochure fig 3

15. Worktable setup example
Worktable Positions
Master Mix Block
Reagent Block
Tips
Tips or Rotor-Gene Q Loading Block
Sample Plate or Rotor-Gene Q Loading Block
Reaction Plate 3 4 1 5 6 2 7 8 3 4 1 6 5 2 7 8

16. Life Can Be So Simple…. QIAgility PCR Setup Wizard
Guides you through creating a
run file with master mix, standard,
Sample and final reaction!

17. Set Up Your Own Run Software Explanation
Just a few simple steps
Define Plate/Tube Format
Sample Bank (number of samples)
Reagents (primer, buffer, taq, etc.)
Master Mix (pre-mix, setup)
Standards
Reactions
Add Controls
Add Standard
Add Samples
GO!

18. View the software